Focal adhesion kinase (FAK) expression and phosphorylation in sea urchin embryos
Focal adhesion kinase (FAK) expression and phosphorylation in sea urchin embryos
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DOI：
10.1016/j.modgep.2003.08.005
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发表时间：
2004-03-01
影响因子：
1.2
通讯作者：
Hille, MB
中科院分区：
文献类型：
--
作者：
García, MG;Toney, SJ;Hille, MB
从海胆Lytechinus variegatus中克隆了3个focal adhesion kinase (FAK) cDNA同工型。海胆的FAK与其他后口动物的FAK的亲缘关系比与无脊椎原口动物或细胞粘附激酶β (cakβ /Pyk2/FAK2)的亲缘关系更密切。FAK在海胆胚胎120细胞期的所有细胞中均有表达，在囊胚中表达强烈。Phospho-FAK集中在早期囊胚上皮细胞的基表面，并出现在初代间充质细胞（PMC）的合胞索中。FAK相关的非激酶结构对FAK的抑制延缓了囊胚膨胀和早期PMC的侵袭。这些结果表明FAK在海胆胚胎细胞粘附和上皮细胞的形状和完整性中起作用。(C) 2003 Elsevier B.V.版权所有
We have cloned three cDNA isoforms of focal adhesion kinase (FAK) from the sea urchin, Lytechinus variegatus. The sea urchin FAK is more closely related to FAK from other deuterostomes than from invertebrate protostomes or to cell adhesion kinase beta (CAKbeta/Pyk2/FAK2). FAK is expressed in all cells of sea urchin embryos by the 120-cell stage and strongly in blastulae. Phospho-FAK concentrates on basal surfaces of epithelial cells in early blastulae and Occurs in syncytial cables of primary mesenchyme cells (PMC). Inhibition of FAK by constructs of FAK-related non-kinase delays blastocoel expansion and early PMC ingression. These results suggest that FAK has roles in cell adhesion and in the shape and integrity of the epithelial cells in sea Urchin embryos. (C) 2003 Elsevier B.V. All rights reserved.