Microtubules tethered at epithelial cell junctions by dynein facilitate efficient junction assembly
Microtubules tethered at epithelial cell junctions by dynein facilitate efficient junction assembly
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DOI：
10.1111/j.1600-0854.2007.00574.x
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发表时间：
2007-07-01
期刊：
影响因子：
4.5
通讯作者：
Holzbaur, Erika L. F.
中科院分区：
文献类型：
--
作者：
Ligon, Lee A.;Holzbaur, Erika L. F.
细胞-细胞粘附的有效重塑在发育和形态发生过程中至关重要。接头组件必须专门和快速地运输到接头组装现场。在这项研究中，我们展示了这种有针对性的贩运可能发生的机制。微管靶向上皮粘附连接，并且在连接组装期间，在接触部位投射和栓系的微管数量增加，这与新生连接处对新材料的需求增加一致。细胞质动力蛋白定位于细胞与细胞接触的部位，微管投射到动力蛋白斑块，在那里它们被束缚。显微注射抗动力蛋白抗体破坏了微管的束缚，表明马达锚定了微管。此外，动力蛋白的破坏抑制连接形成。p120连环蛋白抗体的免疫细胞化学支持这一假设，即拴系微管作为轨道的新组件的交付，以形成连接，提出了一个模型，其中材料的目标是通过动力蛋白拴系微管交付到需要的网站。
Efficient remodeling of cell-cell adhesions is critical during development and morphogenesis. Junctional components must be specifically and rapidly transported to sites of junction assembly. In this study, we show a mechanism by which this targeted trafficking may occur. Microtubules target epithelial adherens junctions, and the number of microtubules both projecting to and tethered at sites of contact is increased during junction assembly, consistent with an increased need for new material at the nascent junction. Cytoplasmic dynein is localized to sites of cell-cell contact, and microtubules project to dynein patches where they become tethered. Microinjection of anti-dynein antibodies disrupts the tethering of microtubules, showing that the motor anchors them. Furthermore, disruption of dynein inhibits junction formation. Immunocytochemistry with antibodies to p120 catenin support the hypothesis that tethered microtubules serve as tracks for delivery of new components to forming junctions, suggesting a model in which material is targeted for delivery to sites of need through microtubules tethered by dynein.