Single Molecule Probe Scanning Optical Force Imaging Microscopefor Viewing Live Cells
Single Molecule Probe Scanning Optical Force Imaging Microscopefor Viewing Live Cells
复制标题
用于观察活细胞的单分子探针扫描光力成像显微镜
DOI：
10.1023/a:1021282504628
复制
发表时间：
2002
影响因子：
1.8
通讯作者：
A. Kusumi
中科院分区：
文献类型：
--
作者：
K. Ritchie;A. Kusumi
我们已经开发了一种成像系统，结合了软顺应性的光阱与单粒子跟踪的灵敏度，使用单分子探针的图像力/活细胞。使用的探针是与单个40 nm胶体金探针缀合的单个（或几个）感兴趣的分子。在活细胞上自由扩散的胶体金/膜蛋白复合物被保持在激光陷阱中，而细胞在下面被扫描。计算机控制允许细胞扫描和探针位置捕获的同步。对探针拖曳的阻力使活细胞膜中的屏障的精细结构成像。
We have developed an imaging system that combines the soft compliance of an optical trap with the sensitivity of single particle tracking to image forces on/in live cells using a single molecule probe. The probe used is a single (or few) molecule of interest that is conjugated with a single 40 nm colloidalgold probe. The colloidal gold/membrane protein complex, freely diffusing on a live cell, is held in a laser trap while the cell is scanned underneath. Computer control allows for synchronization of the cell scan and capture of the probe position. Resistance to the dragging of the probe images a fine structure of barriers in the membrane of live cells.