Approaches to quantifying and visualizing polyelectrolyte multilayer film formation on particles
Approaches to quantifying and visualizing polyelectrolyte multilayer film formation on particles
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DOI：
10.1021/ac060765a
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发表时间：
2006-08-15
影响因子：
7.4
通讯作者：
Caruso, Frank
中科院分区：
文献类型：
--
作者：
Johnston, Angus P. R.;Zelikin, Alexander N.;Caruso, Frank
Colloidal particles prepared by using the layer-by-layer technique are increasingly finding application in diagnostics, drug delivery, and sensing. Herein, we outline methods for applying three established techniques, confocal laser scanning microscopy (CLSM), flow cytometry, and differential interference contrast (DIC) microscopy, to characterize ultrathin films of poly(styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) assembled on silica particles. Both CLSM and flow cytometry require the use of fluorescently labeled polyelectrolytes (PEs). The film homogeneity can be assessed using CLSM, while flow cytometry allows analysis at unparalleled speed (thousands of particles per second) with unprecedented sensitivity (
