Distinct roles of ephrin-B2 forward and ephB4 reverse signaling in endothelial cells
Distinct roles of ephrin-B2 forward and ephB4 reverse signaling in endothelial cells
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DOI：
10.1161/01.atv.0000055440.89758.c2
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发表时间：
2003-02-01
影响因子：
8.7
通讯作者：
Suda, T
中科院分区：
文献类型：
--
作者：
Hamada, K;Oike, Y;Suda, T
目的：跨膜配体ePhin-B2及其受体酪氨酸激酶EphB4分别在动脉和静脉内皮细胞上特异表达，这两种蛋白介导的双向信号在血管发育中起重要作用。然而，这种双向信号是如何引起细胞间黏附或排斥的尚不清楚。方法和结果-使用细胞系和分离的原代内皮细胞，我们证明了通过EphB4受体的EphB4正向信号抑制细胞黏附，而通过跨膜EphB4配体的EphB4反向信号不抑制细胞黏附。EphB4-Fc蛋白对EphB4-Fc蛋白的迁移无明显影响。结论EphB4正向信号转导和EphB4反向信号转导对动、静脉内皮细胞的黏附和迁移有不同的影响。
Objective-The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively, and bidirectional signals mediated by both proteins play an important role in vascular development. However, how such bidirectional signals are required for cell-cell adhesion or repulsion remains unclear.Methods and Results-Using a cell line and sorted primary endothelial cells, we show that ephrin-B2 forward signaling through the EphB4 receptor inhibits cell adhesion, whereas EphB4 reverse signaling by the transmembrane ephrin-B2 ligand does not. Cell migration is also inhibited on immobilized ephrin-B2-Fc but not on EphB4-Fc protein.Conclusions-Ephrin-B2 forward signaling and EphB4 reverse signaling differentially affect cell adhesion and migration between arterial and venous endothelial cells.