A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells
A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells
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DOI：
10.1039/b907515a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Suh, Kahp-Yang
中科院分区：
文献类型：
--
作者：
Jang, Kyung-Jin;Suh, Kahp-Yang
我们开发了一种简单的多层微流控装置，通过集成聚二甲基硅氧烷（PDMS）微流控通道和多孔膜基板培养和分析肾小管细胞。作为模型细胞类型，在通道内培养原代大鼠内髓集合管（IMCD）细胞。为了在体内为细胞产生类似管状的环境，施加1达因/厘米（2）的流体剪切应力5小时，允许培养细胞的最佳流体条件，如通过增强的细胞极化、细胞骨架重组和激素刺激的分子转运所证实的。这些结果表明，这里提出的微流控装置是有用的，类似于在体内的肾小管系统，并在药物筛选和先进的组织工程中具有潜在的应用。
We have developed a simple multi-layer microfluidic device by integrating a polydimethyl siloxane (PDMS) microfluidic channel and a porous membrane substrate to culture and analyze the renal tubular cells. As a model cell type, primary rat inner medullary collecting duct (IMCD) cells were cultured inside the channel. To generate in vivo-like tubular environments for the cells, a fluidic shear stress of 1 dyn/cm(2) was applied for 5 hours, allowing for optimal fluidic conditions for the cultured cells, as verified by enhanced cell polarization, cytoskeletal reorganization, and molecular transport by hormonal stimulations. These results suggest that the microfluidic device presented here is useful for resembling an in vivo renal tubule system and has potential applications in drug screening and advanced tissue engineering.