A rapid determination method for ethylenethiourea in potato and cucumber by modified QuEChERS - high performance liquid chromatography-tandem mass spectrometry.
A rapid determination method for ethylenethiourea in potato and cucumber by modified QuEChERS - high performance liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/j.foodchem.2012.11.038
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发表时间：
2013-06
期刊：
影响因子：
8.8
通讯作者：
Li Zhou;Xiaoliang Liu;Shu Kang;F. Zhang;C. Pan
中科院分区：
文献类型：
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作者：
Li Zhou;Xiaoliang Liu;Shu Kang;F. Zhang;C. Pan
建立了一种快速、灵敏测定马铃薯和黄瓜中乙撑硫脲（ETU）的分析方法。该方法采用改进的QuEChERS，然后进行高效液相色谱-串联质谱（HPLC-MS/MS）分析。ETU经碱性乙腈（含1%NH3.H2O）提取，ZIC-pHILIC色谱柱分离，电喷雾离子源多反应监测（MRM）模式确认。在0.005-0.05mgkg− 1范围内，加标回收率为90.6-103.5%，相对标准偏差（RSD）<7%。方法的检出限（LOD）和定量限（LOQ）分别为0.002mgkg− 1和0.005mgkg−1。并对基体效应和HILIC助留机理进行了评价。最后将该方法应用于马铃薯和黄瓜收获期样品中ETU的检测。在4个黄瓜样品中检测到ETU残留，且残留水平均低于定量限。
A rapid and sensitive analytical method for the determination of ethylenethiourea (ETU) in potatoes and cucumbers is developed. This method employs modified QuEChERS followed by high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) analysis. ETU was extracted by alkaline acetonitrile (containing1%NH3.H20), separated on a ZIC-pHILIC column, confirmed by multiple reaction monitoring (MRM) mode with electrospray ionisation source. This modified procedure showed satisfactory recovery (90.6–103.5%) fortified at the range of 0.005–0.05mgkg−1with relative standard deviation (RSD)<7%. The limits of detection (LOD) and the limits of quantification (LOQ) were 0.002mgkg−1and 0.005mgkg−1, respectively. Matrix effect and HILIC retention mechanism were also evaluated. The method was finally applied to detect ETU in potato and cucumber samples collected at harvest period. Residues of ETU were detected in four cucumber samples with the level lower than LOQ.