Residual dipolar couplings: Synergy between NMR and structural genomics
Residual dipolar couplings: Synergy between NMR and structural genomics
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DOI：
10.1023/a:1013801714041
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Patel, DJ
中科院分区：
文献类型：
--
作者：
Al-Hashimi, HM;Patel, DJ
结构基因组学是对基因产物的重要部分的结构和功能的探索。目前的努力集中在单结构域蛋白质的结构测定，这可以很容易地通过X射线晶体学，NMR光谱和计算同源建模的目标。然而，基因产物与功能的全面关联还需要系统地确定更复杂的蛋白质结构和参与细胞过程的其他生物分子（例如核酸），以及表征与功能相关的生物分子相互作用和动力学。这种核磁共振研究正变得越来越可行，不仅是由于核磁共振方法的最新进展，而且还因为结构基因组学提供了有价值的结构信息和新的实验和计算工具。部分取向系统中残余偶极耦合的测量和其他新的NMR方法将在NMR和结构基因组学之间的这种协同关系中发挥重要作用。核磁共振应用领域的扩展和对未来结构基因组学工作的重要贡献都是可以预期的。
Structural genomics is on a quest for the structure and function of a significant fraction of gene products. Current efforts are focusing on structure determination of single-domain proteins, which can readily be targeted by X-ray crystallography, NMR spectroscopy and computational homology modeling. However, comprehensive association of gene products with functions also requires systematic determination of more complex protein structures and other biomolecules participating in cellular processes such as nucleic acids, and characterization of biomolecular interactions and dynamics relevant to function. Such NMR investigations are becoming more feasible, not only due to recent advances in NMR methodology, but also because structural genomics is providing valuable structural information and new experimental and computational tools. The measurement of residual dipolar couplings in partially oriented systems and other new NMR methods will play an important role in this synergistic relationship between NMR and structural genomics. Both an expansion in the domain of NMR application, and important contributions to future structural genomics efforts can be anticipated.