Sustained expression of the human protooncogene MYCN rescues rat embryo cells from senescence.
Sustained expression of the human protooncogene MYCN rescues rat embryo cells from senescence.
复制标题
人类原癌基因 MYCN 的持续表达可挽救大鼠胚胎细胞的衰老。
DOI：
10.1073/pnas.85.24.9585
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发表时间：
1988
影响因子：
11.1
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Schwab,M;Bishop,JM
人类基因MYCN的扩增可能在人类神经母细胞瘤的恶性进展中发挥作用。为了追求这种可能性，以前的研究表明，MYCN在培养细胞中的大量表达可以引起转化表型的几个方面。我们现在通过证明用MYCN转染的大鼠胚胎细胞可以增殖至少200代来扩展这些发现。建立的细胞的分离依赖于MYCN的高表达和生物选择以消除未转染的细胞。所建立的细胞在同系大鼠或无胸腺小鼠中没有致瘤性，不能在软琼脂中生长，并且在培养中增殖需要相对高浓度的血清。我们的研究结果表明，MYCN的表达增强可以拯救正常细胞免于衰老，增加MYCN作为真实原癌基因的凭据，并确定可用于MYCN表征的额外生物活性。
Amplification of the human gene MYCN may play a role in the malignant progression of human neuroblastomas. In pursuit of this possibility, previous studies have shown that the abundant expression of MYCN in cultured cells can elicit several aspects of the transformed phenotype. We now extend those findings by demonstrating that rat embryo cells transfected with MYCN can proliferate for at least 200 generations. Isolation of established cells was dependent on high expression of MYCN and on biological selection to eliminate untransfected cells. The established cells were not tumorigenic in syngeneic rats or athymic mice, failed to grow in soft agar, and required relatively high concentrations of serum for proliferation in culture. Our results show that enhanced expression of MYCN can rescue normal cells from senescence, add to the credentials of MYCN as an authentic protooncogene, and identify an additional biological activity that can be used in the characterization of MYCN.