Proteins antigenically related to the human erythrocyte glucose transporter in normal and Rous sarcoma virus-transformed chicken embryo fibroblasts.
Proteins antigenically related to the human erythrocyte glucose transporter in normal and Rous sarcoma virus-transformed chicken embryo fibroblasts.
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与正常和劳斯肉瘤病毒转化的鸡胚成纤维细胞中的人红细胞葡萄糖转运蛋白具有抗原相关性的蛋白质。
DOI：
10.1073/pnas.79.5.1540
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发表时间：
1982
影响因子：
11.1
通讯作者：
Weber,MJ
中科院分区：
文献类型：
--
作者：
Salter,DW;Baldwin,SA;Lienhard,GE;Weber,MJ
针对纯化的人红细胞葡萄糖转运蛋白的抗体特异性地从正常和劳斯肉瘤病毒转化的鸡胚胎细胞中沉淀出四种蛋白：一种主要蛋白的Mr为41,000，另一种次要蛋白的Mr为68,000,73,000和82,000。Mr 41,000和82,000蛋白仅在膜组分中发现，而在可溶性组分中没有发现，并且在NaDodSO4/聚丙烯酰胺凝胶电泳上表现出异质迁移，表明糖基化。在恶性转化后，Mr 41,000和82,000蛋白的数量与己糖运输速率的增加成正比，并且增加依赖于src基因产物的表达，正如温度条件src突变体所揭示的那样。我们认为Mr 41,000和82,000蛋白是鸡胚成纤维细胞的葡萄糖转运蛋白，或者是葡萄糖转运蛋白的一个组成部分。这些实验提供了直接证据，表明恶性转化通过增加膜中转运蛋白的数量来增加葡萄糖的转运速率。
Antibody raised against the purified human erythrocyte glucose transporter specifically precipitated four proteins from normal and Rous sarcoma virus-transformed chicken embryo cells: a major protein of Mr 41,000 and minor proteins of Mr 68,000, 73,000, and 82,000. The Mr 41,000 and 82,000 proteins were found only in a membrane fraction, not in the soluble fraction, and displayed a heterogeneous mobility on NaDodSO4/polyacrylamide gel electrophoresis, suggesting glycosylation. The Mr 41,000 and 82,000 proteins were increased in amount after malignant transformation in direct proportion to the increase in hexose transport rate, and the increase was dependent on the expression of the src gene product, as revealed with a temperature-conditional src mutant. We suggest that the Mr 41,000 and 82,000 proteins are the glucose transporter of chicken embryo fibroblasts, or a component of the glucose transporter. These experiments provide direct evidence that malignant transformation increases the rate of glucose transport by increasing the number of transporters in the membrane.