MOLECULAR ANALYSIS OF CYTOPLASMIC MICROTUBULES INSITU - IDENTIFICATION OF BOTH WIDESPREAD AND SPECIFIC PROTEINS
MOLECULAR ANALYSIS OF CYTOPLASMIC MICROTUBULES INSITU - IDENTIFICATION OF BOTH WIDESPREAD AND SPECIFIC PROTEINS
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DOI：
10.1016/0092-8674(81)90516-x
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
SOLOMON, F
中科院分区：
文献类型：
--
作者：
DUERR, A;PALLAS, D;SOLOMON, F
原位分析了几种培养细胞（幼鼠肾BHK细胞、仓鼠成纤维细胞NIL细胞、仓鼠成纤维细胞NIL- hsv细胞、小鼠成纤维细胞3T3细胞、小鼠神经母细胞瘤NB - 2A细胞、大鼠胶质肿瘤C6细胞、人宫颈癌HeLa细胞、人MF - 7细胞和鸡胚成纤维细胞CEF细胞）细胞质微管的分子组成。这种方法使用了含有原始细胞微管的洗涤剂提取的细胞骨架的制剂。微管成分的鉴定基于两个标准；它们在细胞骨架制剂中的存在依赖于完整微管的存在，并且它们通过解聚微管的缓冲液从这些制剂中释放出来。描述符合这些标准的蛋白质。有些局限于某些物种的细胞或原始组织。其他的似乎分布广泛。后者是一种与脑微管蛋白中[小牛脑]tau多肽之一同源的蛋白质。
The molecular components of cytoplasmic microtubules analyzed in situ in several types of cultured cells [baby hamster kidney BHK cell, hamster fibroblast NIL cell, hamster fibroblast NIL-HSV cell, mouse fibroblast 3T3 cell, mouse neuroblastoma NB 2A cell, rat glial tumor C6 cell, human cervical cancer HeLa cell, human MF 7 cell and chicken embryo fibroblast CEF cells.] This approach made use of preparations of detergent-extracted cytoskeletons that contain the original cellular microtubules. Identification of microtubule components is based on 2 criteria; their presence in the cytoskeleton prepations is dependent upon the presence of intact microtubules and they are released from such preparations by buffers that depolymerize microtubules. Proteins that meet these criteria are described. Some are restricted to cells of certain species or tissues of origin. Others appear to be of widespread distribution. Among the latter is a protein homologous to one of the [calf brain] tau polypeptides in brain microtubule protein.