Levels of Adiponectin Expression in Peri-Renal and Subcutaneous Adipose Tissue and Its Determinants in Human Biopsied Samples
Levels of Adiponectin Expression in Peri-Renal and Subcutaneous Adipose Tissue and Its Determinants in Human Biopsied Samples
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DOI：
10.3389/fendo.2019.00897
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发表时间：
2020-02-06
影响因子：
5.2
通讯作者：
Shimabukuro, Michio
中科院分区：
文献类型：
--
作者：
Maimaituxun, Gulinu;Fukuda, Daiju;Shimabukuro, Michio
背景：假设脂肪组织与肾脏的相互作用影响肾功能。此外，肾周脂肪过多可能会增加心脏代谢风险。然而，肾周脂肪脂肪细胞因子的作用（S）从来没有evaluated. Objective. To阐明脂联素在肾周和皮下脂肪组织的表达水平及其决定因素在人类biopsiedsamples.Methods：一对皮下和肾周脂肪组织样本收集80例（男性：54;女性：26）谁接受了泌尿外科手术。在腹部计算机断层扫描上定量皮下脂肪组织（SAT）面积、内脏脂肪组织（VAT）面积和肾周脂肪组织（RAT）体积。通过实时半定量聚合酶链反应（qPCR）评价细胞因子/脂肪细胞因子表达。结果：本研究分析了人血浆脂联素水平的决定因素以及SAT和RAT脂联素表达水平。我们发现：血浆脂联素水平与VAT面积相关，与BMI、腰围、SAT面积、大鼠体积无关，SAT中脂联素表达水平与BMI、腰围、SAT面积相关，与VAT面积、大鼠体积无关;大鼠脂联素的表达水平与BMI、腰围、SAT面积、VAT面积、结论：本研究探讨了泌尿外科手术患者脂联素在大鼠和SAT中的表达水平及其影响因素。大鼠脂联素mRNA水平与SAT和VAT远端脂肪量以及大鼠局部脂肪量呈负相关，而SAT脂联素水平与大鼠体积无关。需要进一步的研究来评估肾周脂肪堆积及其病理生理机制的作用。
Background: The interactions of adipose tissue with the kidney are hypothesized to affect kidney function. Also, excessive peri-renal fat may increase the risk of cardiometabolic risk. However, the role(s) of peri-renal fat adipocytokine has never been evaluated.Objectives: To elucidate levels of adiponectin expression in peri-renal and subcutaneous adipose tissue and its determinants in human biopsied samples.Methods: A pair of subcutaneous and perirenal fat tissue samples were collected from 80 patients (men: 54; women: 26) who underwent urological operations. Subcutaneous adipose tissue (SAT) area, visceral adipose tissue (VAT) area and peri-renal adipose tissue (RAT) volume were quantified on abdominal computed tomography. Cytokine/adipocytokine expression was evaluated by real-time semi-quantitative polymerase chain reaction (qPCR). Probability was considered significant if P < 0.05.Results: Current study evaluated determinants of plasma adiponectin levels and expression levels of adiponectin in SAT and RAT in human samples. We found that: first, plasma adiponectin levels were correlated with VAT area but not with BMI, waist circumference, SAT area, and RAT volume; second, expression levels of adiponectin in SAT were correlated with BMI, waist circumference, and SAT area but not with VAT area and RAT volume; and third, expression levels of adiponectin in RAT were correlated with all adiposity indices including BMI, waist circumference, SAT area, VAT area, and RAT volume.Conclusion: This study evaluated levels of adiponectin expression in RAT and SAT and its determinants in patients who underwent urological operation. Levels of adiponectin mRNA in RAT were negatively correlated with remote fat mass in SAT and VAT and also with local fat mass in RAT, while level of adiponectin in SAT was not correlated with RAT volume. Further studies are warranted to evaluate roles of peri-renal fat mass accumulation and its pathophysiological machineries.