Chromosome substitution strains: gene discovery, functional analysis, and systems studies.
Chromosome substitution strains: gene discovery, functional analysis, and systems studies.
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DOI：
10.1007/s00335-012-9426-y
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发表时间：
2012-10
期刊：
影响因子：
2.5
通讯作者：
Shiroishi, Toshihiko
中科院分区：
文献类型：
--
作者：
Nadeau, Joseph H.;Forejt, Jiri;Takada, Toyoyuki;Shiroishi, Toshihiko
实验室小鼠在生物医学研究中很有价值，部分原因是遗传资源的非凡多样性，可用于研究复杂的遗传特征，并作为人类生物学和疾病的模型。染色体替代菌株（CSS）在这一资源组合中很重要，因为它们已被证明可用于基因发现、遗传和表观遗传研究、功能表征和系统分析。通过用来自供体菌株的相应染色体替换宿主菌株中的单个染色体来制备CSS。一个完整的CSS面板涉及总共22个工程近交系，19个常染色体中的每一个，X和Y染色体各一个，线粒体一个。基因组调查简单地涉及将每个CSS的每个表型与宿主菌株的表型进行比较。可用于实验室小鼠的CSS面板已被用于解剖各种各样的表型，并表征一系列令人印象深刻的疾病模型。这些调查揭示了相当大的表型多样性，即使在密切相关的祖株，证据强上位性和遗传的表观遗传变化。也许最重要的是，大概是因为它们独特的遗传组成，CSS，和同源菌株从它们衍生的数量性状基因座（QTL）的遗传变异很容易识别和功能特征。总之，这些研究表明，CSS是实验室小鼠的重要资源。
Laboratory mice are valuable in biomedical research in part because of the extraordinary diversity of genetic resources that are available for studies of complex genetic traits and as models for human biology and disease. Chromosome substitution strains (CSSs) are important in this resource portfolio because of their demonstrated use for gene discovery, genetic and epigenetic studies, functional characterizations, and systems analysis. CSSs are made by replacing a single chromosome in a host strain with the corresponding chromosome from a donor strain. A complete CSS panel involves a total of 22 engineered inbred strains, one for each of the 19 autosomes, one each for the X and Y chromosomes, and one for mitochondria. A genome survey simply involves comparing each phenotype for each of the CSSs with the phenotypes of the host strain. The CSS panels that are available for laboratory mice have been used to dissect a remarkable variety of phenotypes and to characterize an impressive array of disease models. These surveys have revealed considerable phenotypic diversity even among closely related progenitor strains, evidence for strong epistasis and for heritable epigenetic changes. Perhaps most importantly, and presumably because of their unique genetic constitution, CSSs, and congenic strains derived from them, the genetic variants underlying quantitative trait loci (QTLs) are readily identified and functionally characterized. Together these studies show that CSSs are important resource for laboratory mice.
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影响因子：
64.5
作者：
Carone BR;Fauquier L;Habib N;Shea JM;Hart CE;Li R;Bock C;Li C;Gu H;Zamore PD;Meissner A;Weng Z;Hofmann HA;Friedman N;Rando OJ
通讯作者：
Rando OJ
影响因子：
2.5
作者：
Churchill, Gary A.;Gatti, Daniel M.;Munger, Steven C.;Svenson, Karen L.
通讯作者：
Svenson, Karen L.
影响因子：
11.2
作者：
Anderson, Philip D.;Nelson, Vicki R.;Nadeau, Joseph H.
通讯作者：
Nadeau, Joseph H.
影响因子：
4.6
作者：
Ackerman, KG;Huang, HL;Beier, DR
通讯作者：
Beier, DR
影响因子：
6.7
作者：
Ahn SH;Deshmukh H;Johnson N;Cowell LG;Rude TH;Scott WK;Nelson CL;Zaas AK;Marchuk DA;Keum S;Lamlertthon S;Sharma-Kuinkel BK;Sempowski GD;Fowler VG Jr
通讯作者：
Fowler VG Jr