Regulation of singlet oxygen-induced apoptosis by cytosolic NADP+-dependent isocitrate dehydrogenase
Regulation of singlet oxygen-induced apoptosis by cytosolic NADP+-dependent isocitrate dehydrogenase
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DOI：
10.1007/s11010-007-9421-x
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发表时间：
2007-08-01
影响因子：
4.3
通讯作者：
Park, Jeen-Woo
中科院分区：
文献类型：
--
作者：
Kim, Sun Yee;Lee, Su Min;Park, Jeen-Woo
单线氧是一种高反应性的分子氧形式，它可能通过氧化关键细胞大分子来损害生命系统，并且还促进了有害过程，例如细胞死亡。最近，我们证明了氧化还原平衡和针对氧化损伤的细胞防御是胞质NADP（+） - 依赖性异位酸异位酸盐脱氢酶（IDPC）的主要功能，通过为抗氧化剂系统提供NADPH。在本报告中，我们证明了HL-60细胞中IDPC活性的调节调节氧气诱导的凋亡。当我们检查IDPC抵抗单线氧诱导的单重氧诱导的凋亡的保护作用时，用cDNA转染的HL-60细胞在有义务和反义方向上用于小鼠IDPC，观察到在靶细胞中表达的IDPC量与其敏感性及其易感性之间存在明显的反相关关系。凋亡。结果表明，IDPC在单线氧诱导的HL-60细胞凋亡中起重要的保护作用。
Singlet oxygen is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules and it also promotes deleterious processes such as cell death. Recently, we demonstrated that the control of redox balance and the cellular defense against oxidative damage are the primary functions of cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) through supplying NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPc activity in HL-60 cells regulates singlet oxygen-induced apoptosis. When we examined the protective role of IDPc against singlet oxygen-induced apoptosis with HL-60 cells transfected with the cDNA for mouse IDPc in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPc expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPc plays an important protective role in apoptosis of HL-60 cells induced by singlet oxygen.