"Dipstick" Colorimetric Detection of Metal Ions Based on Immobilization of DNAzyme and Gold Nanoparticles onto a Lateral Flow Device.
"Dipstick" Colorimetric Detection of Metal Ions Based on Immobilization of DNAzyme and Gold Nanoparticles onto a Lateral Flow Device.
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DOI：
10.1007/978-1-4939-6848-0_24
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发表时间：
2017
影响因子：
--
通讯作者：
Debapriya Mazumdar;Tian Lan;Yi Lu
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文献类型：
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作者：
Debapriya Mazumdar;Tian Lan;Yi Lu
实时、现场检测和量化不同的痕量分析物是一项挑战，需要搜索一类通用的分子来识别广泛的污染物，并将这种识别转换为易于检测的信号。功能核酸，包括DNAzyme(具有催化活性的DNA)和适配子(与分析物结合的核酸)，是目标识别的理想候选者。这些核酸可以通过一种称为体外选择的组合生物学方法来选择，以与特定的分析物相互作用，具有高度的特异性和敏感性。此外，通过连接信号分子，它们可以被整合到传感器中。由于高消光系数和与距离相关的光学性质，金属纳米粒子，如常用的金纳米粒子，在将分析物特定的功能DNA转化为颜色传感器方面非常有吸引力。DNAzyme定向组装的金纳米颗粒已被用于制造铅、铀和铜等金属离子的比色传感器。为了使操作更容易，更不容易受到操作员错误的影响，已经建立了试纸测试。在这里，我们描述了制备DNAzyme连接的金纳米颗粒(AuNP)的方案，然后将其固定在横向流动设备上，以进行易于使用的金属离子试纸测试。
Real-time, on-site detection and quantification of different trace analytes is a challenge that requires both searching a general class of molecules to recognize a broad range of contaminants and translating this recognition to easily detectable signals. Functional nucleic acids, which include DNAzymes (DNA with catalytic activity) and aptamers (nucleic acids that bind an analyte), are ideal candidates for the target recognition. These nucleic acids can be selected by a combinatorial biology method called in vitro selection to interact with a particular analyte with high specificity and sensitivity. Furthermore, they can be incorporated into sensors by attaching signaling molecules. Due to the high extinction coefficients and distance-dependent optical properties, metallic nanoparticles such as the commonly used gold nanoparticles have been shown to be very attractive in converting analyte-specific functional DNA into colorimetric sensors. DNAzyme directed assembly of gold nanoparticles has been used to make colorimetric sensors for metal ions such as lead, uranium, and copper. To make the operation even easier and less vulnerable to operator's errors, dipstick tests have been constructed. Here, we describe protocols for the preparation of DNAzyme-linked gold nanoparticles (AuNP) that are then immobilized on to lateral flow devices to make easy-to-use dipstick tests for metal ions.