Evaluation of genetic diversity in Piper spp using RAPD and SRAP markers
Evaluation of genetic diversity in Piper spp using RAPD and SRAP markers
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使用 RAPD 和 SRAP 标记评估 Piper 属遗传多样性
DOI：
10.4238/2011.november.29.4
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发表时间：
2011-01-01
影响因子：
0.4
通讯作者：
Liu, J. -P.
中科院分区：
文献类型：
--
作者：
Jiang, Y.;Liu, J. -P.
对海南岛74株胡椒属植物进行了随机扩增多态DNA(RAPD)和序列相关扩增多态(SRAP)分析。结果表明，在胡椒属植物遗传多样性研究中，SRAP技术可能比随机扩增多态DNA技术信息量更大，效率更高。海南胡椒种质资源的遗传多样性水平较高，平均香农多样性指数分别为0.2822和0.2909，平均遗传多样性指数分别为0.1880和0.1947。74株胡椒属植物的遗传相似系数范围分别为0.486~0.991和0.520~1.000(平均遗传距离分别为0.505和0.480)，种内遗传距离分别为0.063~0.291和0.096~0.234。这些遗传指数表明，这些物种在遗传上关系密切。利用随机扩增多态性DNA标记构建的树状图与基于SRAP标记的树状图在拓扑结构上有所不同，但SRAP技术可以清晰地将所有胡椒属区分开来。对6个居群遗传变异水平的评价表明，尖峰岭居群和吊罗山居群的有效等位基因数、Nei‘s基因多样性和Shannon信息指数均高于其他居群，因此应优先保护这两个居群的野生资源。
Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analysis were applied to 74 individual plants of Piper spp in Hainan Island. The results showed that the SRAP technique may be more informative and more efficient and effective for studying genetic diversity of Piper spp than the RAPD technique. The overall level of genetic diversity among Piper spp in Hainan was relatively high, with the mean Shannon diversity index being 0.2822 and 0.2909, and the mean Nei's genetic diversity being 0.1880 and 0.1947, calculated with RAPD and SRAP data, respectively. The ranges of the genetic similarity coefficient were 0.486-0.991 and 0.520-1.000 for 74 individual plants of Piper spp (the mean genetic distance was 0.505 and 0.480) and the within-species genetic distance ranged from 0.063 to 0.291 and from 0.096 to 0.234, estimated with RAPD and SRAP data, respectively. These genetic indices indicated that these species are closely related genetically. The dendrogram generated with the RAPD markers was topologically different from the dendrogram based on SRAP markers, but the SRAP technique clearly distinguished all Piper spp from each other. Evaluation of genetic variation levels of six populations showed that the effective number of alleles, Nei's gene diversity and the Shannon information index within Jianfengling and Diaoluoshan populations are higher than those elsewhere; consequently conservation of wild resources of Piper in these two regions should have priority.