Analysis of cholesteryl esters and diacylglycerols using lithiated adducts and electrospray ionization-tandem mass spectrometry.
Analysis of cholesteryl esters and diacylglycerols using lithiated adducts and electrospray ionization-tandem mass spectrometry.
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DOI：
10.1016/j.ab.2011.06.015
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发表时间：
2011-10-15
影响因子：
2.9
通讯作者：
Ford DA
中科院分区：
文献类型：
--
作者：
Bowden JA;Albert CJ;Barnaby OS;Ford DA
胆固醇酯（CE）和甘油二酯（DAG）是重要的脂质储存和信号分子。这些脂质的质谱分析是复杂的，这些脂质类共有的同量异位分子离子的存在下，并通过相对较差的电喷雾电离，这是一个固有的弱偶极矩在这些脂质类的结果。本研究表明，CE和DAG分子离子的锂化加合物在MS/MS扫描模式中具有增强的电离和脂质类特异性片段化，从而允许实施能够进行脂质类特异性检测的策略。使用中性损失模式（用于胆固醇酯（NL 368.5）中胆甾烷的损失）和DAG分子种类的特定选择反应监测，测定特定分子种类对内标物的响应。在与棕榈酸和油酸孵育的人冠状动脉内皮细胞（HCAEC）中定量CE和DAG分子种类。此外，NL 368.5光谱揭示了胆固醇酯分子种类的脂肪族脂肪酸残基的氧化。总之，这些研究证明了一种新的分析方法，以评估CE和DAG分子种类，利用锂化加合物的效用与MS/MS方法。
Cholesteryl ester (CE) and diacylglycerol (DAG) molecular species are important lipid storage and signaling molecules. Mass spectrometric analyses of these lipids are complicated by the presence of isobaric molecular ions shared by these lipid classes, and by relatively poor electrospray ionization, which is a consequence of an inherent weak dipole moment in these lipid classes. The present study demonstrates that lithiated adducts of CE and DAG molecular ions have enhanced ionization and lipid class specific fragmentation in MS/MS scan modes, thus allowing the implementation of strategies capable of lipid class specific detection. Using neutral loss mode for the loss of cholestane from cholesterol esters (NL 368.5) and specific selected reaction monitoring for DAG molecular species, the response of specific molecular species to that of internal standards was determined. CE and DAG molecular species were quantified in human coronary artery endothelial cells (HCAEC) incubated with both palmitic and oleic acid. Furthermore, NL 368.5 spectra revealed the oxidation of the aliphatic fatty acid residues of cholesterol ester molecular species. Taken together, these studies demonstrate a new analytical approach to assess CE and DAG molecular species that exploits the utility of lithiated adducts in conjunction with MS/MS approaches.
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
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