A MARKER-FREE ALIGNMENT METHOD FOR ELECTRON TOMOGRAPHY
A MARKER-FREE ALIGNMENT METHOD FOR ELECTRON TOMOGRAPHY
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DOI：
10.1016/0304-3991(95)00006-m
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发表时间：
1995-06-01
期刊：
影响因子：
2.2
通讯作者：
FRANK, J
中科院分区：
文献类型：
--
作者：
LIU, Y;PENCZEK, PA;FRANK, J
在生物标本的电子层析成像中，通常使用基准标记来实现输入投影的精确对准。我们解决了自由支承且不允许使用标记的物体的投影对齐问题。为此，我们提出了一种新的基于傅里叶空间共线原理的单轴倾斜几何对准算法。为了克服共线法的噪声敏感性，提出了一种迭代格式。该算法用于对不能与基准标记进行比对的数据集进行比对。
In electron tomography of biological specimens, fiducial markers are normally used to achieve accurate alignment of the input projections. We address the problem of alignment of projections from objects that are freely supported and do not permit the use of markers. To this end we present a new alignment algorithm for single-axis tilt geometry based on the principle of Fourier-space common lines. An iterative scheme has been developed to overcome the noise-sensitivity of the common-line method. This algorithm was used to align a data set that was not amenable to alignment with fiducial markers.