Studies on immunity in anthrax. III. Elaboration of protective antigen in a chemically defined, non-protein medium.
Studies on immunity in anthrax. III. Elaboration of protective antigen in a chemically defined, non-protein medium.
复制标题
炭疽病的免疫研究。
作者：
G. Wright;M. Hedberg;J. B. Slein
炭疽芽孢杆菌保护性抗原是在由17种氨基酸、无机盐、腺嘌呤、鸟嘌呤、尿嘧啶、硫胺素、谷氨酰胺、葡萄糖和碳酸氢钠组成的化学定义的非蛋白质介质中获得的。炭疽杆菌非蛋白水解性突变株静态培养20小时的无菌滤液，当分次注射总计1毫升的剂量时，能够免疫兔子，使它们在10,000个致命炭疽杆菌孢子的皮内攻击中存活下来，相当于至少100个致死剂量。这些制剂使豚鼠免疫抵抗1000个孢子的攻击，当用明矾沉淀时，在小鼠中产生了可检测到的免疫力。初步实验表明，该抗原也能在猴子身上产生有效的免疫力。获得了生产抗原的一些关键成分和培养条件的初步迹象。粗培养滤液的保护性抗原活性相对不稳定，但保护性抗原可以通过冷冻干燥或明矾沉淀来浓缩和稳定。讨论了这一结果的意义。
Summary Elaboration of the protective antigen of Bacillus anthracis was obtained in a chemically-defined, non-protein medium composed of 17 amino acids, inorganic salts, adenine, guanine, uracil, thiamine, glutamine, glucose, and sodium bicarbonate. Sterile filtrates of static 20-hour cultures of a non-proteolytic mutant strain of B. anthracis , when injected in divided doses totaling 1 ml., were capable of immunizing rabbits so that they survived intracutaneous challenge with 10,000 spores of virulent B. anthracis , representing at least 100 lethal doses. The preparations immunized guinea pigs against a challenge of 1000 spores and, when precipitated with alum, produced detectable immunity in mice. A preliminary experiment suggested that the antigen also produced effective immunity in monkeys. Tentative indications as to some of the critical constituents and cultural conditions for production of antigen were obtained. Protective antigen activity of the crude culture filtrate was relatively unstable; the protective antigen could, however, be concentrated and stabilized by lyophilization or by alum precipitation. The meaning of the results is discussed.