A detailed analysis of the leaf rolling mutant sll2 reveals complex nature in regulation of bulliform cell development in rice (Oryza sativa L.)
A detailed analysis of the leaf rolling mutant sll2 reveals complex nature in regulation of bulliform cell development in rice (Oryza sativa L.)
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DOI：
10.1111/plb.12255
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发表时间：
2015-03-01
期刊：
影响因子：
3.9
通讯作者：
Wan, J-M.
中科院分区：
文献类型：
--
作者：
Zhang, J-J.;Wu, S-Y.;Wan, J-M.
泡状细胞是一种体积大、壁薄、高度液泡化的细胞，在干旱和高温条件下控制叶片卷曲起着重要作用。然而，调节泡状细胞发育的分子机制还没有得到很好的证明。在这里，我们报告的分离和表征的水稻卷叶突变体，命名为葱样2（sll2）。从第六叶期开始，sll2植物表现出正面卷起的叶，伴随着增加的光合作用和降低的株高和分蘖数。组织学分析显示泡状细胞收缩，导致叶片向内弯曲。该突变体为隐性突变体，回复突变率为9%。卷叶是由T-DNA插入引起的。使用TAIL-PCR克隆插入片段，结果表明T-DNA插入到LOC_0s07g38664的启动子区域。出乎意料的是，通过T-DNA中的35S增强子增强的LOC_0s07g38664的表达不负责卷叶表型。此外，增强子还发挥了长距离效应，包括上调几个泡状细胞相关基因。在sll2oul1双突变体中，sll2抑制oul1的叶片向外卷曲。我们认为sll2的卷叶可能是多基因共同作用的结果，这意味着泡状细胞发育的调控是一个复杂的网络。
Bulliform cells are large, thin-walled and highly vacuolated cells, and play an important role in controlling leaf rolling in response to drought and high temperature. However, the molecular mechanisms regulating bulliform cell development have not been well documented. Here, we report isolation and characterisation of a rice leaf-rolling mutant, named shallot-like 2 (sll2). The sll2 plants exhibit adaxially rolled leaves, starting from the sixth leaf stage, accompanied by increased photosynthesis and reduced plant height and tiller number. Histological analyses showed shrinkage of bulliform cells, resulting in inward-curved leaves. The mutant is recessive and revertible at a rate of 9%. The leaf rolling is caused by a T-DNA insertion. Cloning of the insertion using TAIL-PCR revealed that the T-DNA was inserted in the promoter region of LOC_Os07g38664. Unexpectedly, the enhanced expression of LOC_Os07g38664 by the 35S enhancer in the T-DNA is not responsible for the leaf rolling phenotype. Further, the enhancer also exerted a long-distance effect, including up-regulation of several bulliform cell-related genes. sll2 suppressed the outward leaf rolling of oul1 in the sll2oul1 double mutant. We conclude that leaf rolling in sll2 could be a result of the combined effect of multi-genes, implying a complex network in regulation of bulliform cell development.