Glutathione S Transferase Activity in Indian Vectors of Malaria: A Defense Mechanism Against DDT
Glutathione S Transferase Activity in Indian Vectors of Malaria: A Defense Mechanism Against DDT
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DOI：
10.1603/me10194
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发表时间：
2011-05-01
影响因子：
2.1
通讯作者：
Jambulingam, P.
中科院分区：
文献类型：
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作者：
Gunasekaran, K.;Muthukumaravel, S.;Jambulingam, P.
谷胱甘肽S转移酶（GSTs）是一种多功能酶，在大多数昆虫类群中参与对外源化合物的解毒。昆虫GSTs的意义在于其活性水平的升高与杀虫剂抗性的关系。本研究旨在探讨印度两种ddt耐药疟疾媒介——culicifacies按蚊和环状按蚊，以及一种ddt敏感媒介——河流按蚊中GSTs的代谢状况。奥里萨邦Malkangiri和Koraput地区是恶性疟疾的流行地区，有很长的杀虫剂喷洒历史，是研究区域。从野生捕获的三种载体雌性中培养F-1后代，并进行生化试验以检测gst介导的DDT抗性机制。酶分析结果显示，抗ddt的甘油三酯活性显著增加3倍。与易感人群进行比较。在抗ddt的An。结果表明，GST活性的中位数（71.8 mu mol/min/mg）与抗ddt品种的估计值基本一致。（74.6 mu mol/min/mg）。culicifacies可能是DDT解毒的高水平。此外，抗ddt菌株的GST活性也有所提高。文化相与文化。ddt易感地区的年轮病显著高于其他地区。其GST活性为20.0 μ mol/min/mg。此外，通过比较野生雌鱼及其F-1后代的GST活性，证实了GST介导的DDT解毒作用。
Glutathione S transferases (GSTs) are multifunctional enzymes involved in detoxification of xenobiotic compounds in majority of the insect groups. Significance of insect GSTs is their elevated level of activity in association with insecticide resistance. This investigation was to explore the metabolic status of GSTs in two Indian DDT-resistant malaria vectors, Anopheles culicifacies and Anopheles annularis, and one DDT-susceptible vector, Anopheles fluviatilis. Malkangiri and Koraput districts of Orissa State, endemic for falciparum malaria and having a long insecticide spraying history, were the study areas. F-1 progeny was raised from wild-caught females of the three vectors and used for biochemical assays to detect the GST-mediated DDT resistance mechanism. Results of the enzyme assay showed a significant 3-fold increase in GST activity in DDT-resistant An. annularis compared with its susceptible population. In DDT-resistant An. culicifacies, the median GST activity (71.8 mu mol/min/mg) was almost the same as estimated in the DDT-resistant An. annularis (74.6 mu mol/min/mg), suggesting that the GST activity estimated in An. culicifacies could be an elevated level for detoxification of DDT. Furthermore, the GST activity in DDT-resistant An. culicifacies and An. annularis was significantly higher than that in the DDT-susceptible An. fluviatilis, which had a GST activity of 20.0 mu mol/min/mg. Also, the GST-mediated DDT detoxification was confirmed by comparing GST activity in wild-caught females with that in their F-1 progeny.