Establishment of a green fluorescent protein tracing murine model focused on the functions of host components in necrosis repair and the niche of subcutaneously implanted glioma
Establishment of a green fluorescent protein tracing murine model focused on the functions of host components in necrosis repair and the niche of subcutaneously implanted glioma
复制标题
建立绿色荧光蛋白示踪小鼠模型，重点关注宿主成分在坏死修复中的功能和皮下植入神经胶质瘤的生态位。
DOI：
10.3892/or.2013.2873
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发表时间：
2014-02-01
期刊：
影响因子：
4.2
通讯作者：
Huang, Qiang
中科院分区：
文献类型：
--
作者：
Lu, Zhao-Hui;Lv, Ke;Huang, Qiang
由于胶质瘤干细胞和胶质瘤生态位研究的进展，有必要开发一种动物模型，以便于阐明宿主组织和细胞的作用。本研究旨在建立绿色荧光蛋白裸鼠皮下移植瘤模型，并利用该模型分析宿主细胞在肿瘤坏死修复中的作用。将来源于人神经胶质瘤干/祖细胞系SU 3的肿瘤皮下植入绿色荧光蛋白裸鼠中。然后将植入的肿瘤在动物之间传递10代。最后，用传统病理学、免疫病理学技术和荧光照相术检测皮下异种移植物。每代的成瘤率均为100%。皮下移植瘤血管丰富，坏死出血灶高表达缺氧诱导因子-1 α、肿瘤坏死因子、Ki-67、CD 68和CD 11b。在间质组织中，特别是在陈旧性出血灶中，有大量表达绿色荧光蛋白、CD 68和CD 11 b的细胞。绿色荧光蛋白裸小鼠皮下移植瘤不仅保持了胶质瘤干/祖细胞的高侵袭性和致瘤性，而且由高浓度的肿瘤血管和坏死出血灶组成。皮下异种移植物也表达高水平的肿瘤微环境相关蛋白和宿主源性肿瘤间质分子。该模型对进一步研究肿瘤组织重塑和肿瘤微环境具有重要的潜力。
Due to progress in the research of glioma stem cells and the glioma niche, development of an animal model that facilitates the elucidation of the roles of the host tissue and cells is necessary. The aim of the present study was to develop a subcutaneous xenograft green fluorescent protein nude mouse model and use this model to analyze the roles of host cells in tumor necrosis repair. Tumors derived from the human glioma stem/progenitor cell line SU3 were subcutaneously implanted in green fluorescent protein nude mice. The implanted tumors were then passed from animal to animal for 10 generations. Finally, subcutaneous xenografts were assayed with traditional pathology, immunopathological techniques and fluorescence photography. For each generation, the tumorigenicity rate was 100%. Subcutaneous xenografts were rich in blood vessels, and necrotic and hemorrhagic foci, which highly expressed hypoxia-inducible factor-1α, tumor necrosis factor, Ki-67, CD68 and CD11b. In the interstitial tissue, particularly in old hemorrhagic foci, there were numerous cells expressing green fluorescent protein, CD68 and CD11b. Green fluorescent protein nude mouse subcutaneous xenografts not only consistently maintained the high invasiveness and tumorigenicity of glioma stem/progenitor cells, but also consisted of a high concentration of tumor blood vessels and necrotic and hemorrhagic foci. Subcutaneous xenografts also expressed high levels of tumor microenvironment-related proteins and host-derived tumor interstitial molecules. The model has significant potential for further research on tumor tissue remodeling and the tumor microenvironment.