Expression patterns of cell-type-specific genes in Dictyostelium
Expression patterns of cell-type-specific genes in Dictyostelium
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DOI：
10.1091/mbc.12.9.2590
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发表时间：
2001-09-01
影响因子：
3.3
通讯作者：
Loomis, WF
中科院分区：
文献类型：
--
作者：
Iranfar, N;Fuller, D;Loomis, WF
通过使用由富含前茎和前孢子细胞的级分制备的探针询问携带盘基网柄菌基因片段的微阵列来识别细胞类型特异性基因。 Northern 分析证实了 17 个新鉴定基因的 mRNA 的细胞类型特异性积累。携带 690 个靶标的 DNA n-deroarrays 用于确定开发过程中的表达谱。这些曲线符合基于生物学的动力学方程，以提取转录起始和终止的时间。虽然在蛞蝓阶段细胞类型富集的大多数基因首先在聚集体中分选出的前孢子和前茎细胞时表达，但发现有些基因甚至在细胞聚集之前就表达了。这些早期基因可能最初在所有细胞中表达，然后优先在一种或另一种细胞类型中表达。或者，细胞类型分化可能在发育开始后不久就开始。
Cell-type specific genes were recognized by interrogating microarrays carrying Dictyostelium gene fragments with probes prepared from fractions enriched in prestalk and prespore cells. Cell-type specific accumulation of mRNA from 17 newly identified genes was confirmed by Northern analyses. DNA n-deroarrays carrying 690 targets were used to determine expression profiles during development. The profiles were fit to a biologically based kinetic equation to extract the times of transcription onset and cessation. Although the majority of the genes that were cell-type enriched at the slug stage were first expressed as the prespore and prestalk cells sorted out in aggregates, some were found to be expressed earlier before the cells had even aggregated. These early genes may have been initially expressed in all cells and then preferentially turned over in one or the other cell type. Alternatively, cell type divergence may start soon after the initiation of development.