Pigmentation and enzymic inhibition by carbonyl compounds in the rabbit lens.
Pigmentation and enzymic inhibition by carbonyl compounds in the rabbit lens.
复制标题
兔晶状体中羰基化合物的色素沉着和酶抑制。
DOI：
10.1016/0006-2952(72)90176-1
复制
发表时间：
1972
影响因子：
5.8
通讯作者：
O. Montgomery
中科院分区：
文献类型：
--
作者：
T. Scharff;O. Montgomery
当某些α-羟基羰基或二羰基化合物添加到培养的兔晶状体中时，酶活性、颜色和荧光发生变化。这些变化类似于新陈代谢的降低以及在某些老年人类晶状体中看到的颜色和荧光的发展。一种代表性化合物乙醇醛抑制至少一种酶（醛缩酶）的活性。当添加到赖氨酸、肌肉醛缩酶、透析晶状体匀浆或完整的培养兔晶状体中时，乙醇醛会引发紫外线荧光和颜色，后者的范围逐渐从黄色到棕色甚至到黑色。乙醇醛与赖氨酸在 pH 8 的溶液中形成 1-1 加合物。通过随后的美拉德型反应，发生了进一步的变化，产生了类似于乙醇醛处理的镜片或有色人类镜片中所见的颜色渐变。其他抑制晶状体代谢的α-羟基羰基化合物或二羰基化合物（T. G. Scharff 和 O. L. Montgomery, Proc. Soc. exp. Biol. Med.134, 658 1970）也可能与溶液中的氨基酸发生美拉德型反应，与肌肉醛缩酶、透析晶状体匀浆或完整晶状体形成类似颜色的荧光产物。未鉴定的羰基化合物与游离或蛋白质掺入的氨基酸之间的有色美拉德型复合物可能与衰老的人类晶状体或其他色素老化组织的新陈代谢减少和颜色形成有关。
When certain α-hydroxycarbonyl or dicarbonyl compounds were added to cultured rabbit lenses, changes occurred in enzymic activity, color and fluorescence. These changes resemble the decreased metabolism and the development of color and fluorescence that is seen in some aged human lenses. One representative compound, glycolaldehyde, inhibited activity of at least one enzyme (aldolase). When added to lysine, to muscle aldolase, to dialyzed lens homogenates or to intact cultured rabbit lenses, glycolaldehyde elicited u.v.-fluorescence and color, the latter ranging progressively from yellow to brown or even to black. Glycolaldehyde in solution with lysine at pH 8 formed a 1-1 adduct. Through subsequent reactions of the Maillard type there occurred further changes yielding a progression of color similar to that seen in glycolaldehyde-treated lenses or in colored human lenses. Othera-hydroxycarbonyl compounds or dicarbonyls which are inhibitory to metabolism in the lens (T. G. Scharff and O. L. Montgomery,Proc. Soc. exp. Biol. Med.134, 658 1970) also probably underwent Maillard-type reactions with amino acids in solution to form similarly colored, fluorescent products with muscle aldolase, dialyzed lens homogenates or intact lenses. It is possible that colored Maillard-type complexes between unidentified carbonyl compounds and free or protein-incorporated amino acids may be involved in reduced metabolism and in color development in the aged human lens or other pigmented, aging tissues.