The hSNM1 protein is a DNA 5-exonuclease
The hSNM1 protein is a DNA 5-exonuclease
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DOI：
10.1093/nar/gkm530
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发表时间：
2007-09-01
影响因子：
14.9
通讯作者：
Moses, Robb
中科院分区：
文献类型：
--
作者：
Hejna, James;Philip, Sahaayaruban;Moses, Robb
人SNM 1蛋白是催化核酸底物水解的高度保守蛋白质组的成员。虽然在哺乳动物细胞中过量生产是不稳定的，但我们已经在昆虫细胞系统中过量生产了重组hSNM 1蛋白。该蛋白质是一种单链5 '-核酸外切酶，就像它的酵母同系物一样。该酶利用DNA或RNA底物，需要5 '-磷酸部分，对双链底物显示出非常小的活性，并且以与单体一致的大小起作用。核酸外切酶活性需要保守的β-内酰胺酶结构域;保守天冬氨酸的定点诱变使核酸外切酶失活。
The human SNM1 protein is a member of a highly conserved group of proteins catalyzing the hydrolysis of nucleic acid substrates. Although overproduction is unstable in mammalian cells, we have overproduced a recombinant hSNM1 protein in an insect cell system. The protein is a single-strand 5'-exonuclease, like its yeast homolog. The enzyme utilizes either DNA or RNA substrates, requires a 5'-phosphate moiety, shows very little activity on double-strand substrates, and functions at a size consistent with a monomer. The exonuclease activity requires the conserved beta-lactamase domain; site-directed mutagenesis of a conserved aspartate inactivates the exonuclease.