High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 3. Effect of electrical field shape.
High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 3. Effect of electrical field shape.
复制标题
DNA 脉冲场凝胶电泳中的高分辨率分离和精确尺寸测定。
DOI：
10.1021/bi00426a021
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Smith,CL
中科院分区：
文献类型：
--
作者：
Cantor,CR;Gaal,A;Smith,CL
哥伦比亚大学内科和外科医生学院遗传与发育、微生物学和精神病学系，纽约，纽约10032，和LKB Produkter AB，Bromma，瑞典，1988年2月23日接收;修订版手册，1988年7月26日接收摘要：脉冲场凝胶电泳的分辨率受所用电极的数量和配置的显著影响，因为这些改变了所施加的电场的形状。在这里，我们提出了最常用的脉冲场凝胶电泳配置之一的电极位置的效果的计算和实验。目的是探索电场形状的哪些方面与改进的电泳分辨率相关。最关键的变量似乎是交替电场之间的角度。最有效的电极配置产生大于110的角度。场之间不断增加的角度产生条带锐化，从而大大增强了分辨率。
Departments of Genetics and Development, Microbiology, and Psychiatry, College of Physicians and Surgeons, Columbia University, New York, New York 10032, and LKB Produkter AB, Bromma, Sweden Received February 23, 1988; Revised Manuscript Received July 26, 1988 abstract: The resolution of pulsed-field gel electrophoresis is dramatically affected by the number and configuration of theelectrodes used, because these alter the shape of the applied electrical fields. Here we present calculations and experiments on the effect of electrode position in one of the most commonly used pulsed-field gel electrophoresis configurations. The goal was to explore which aspects of the electrical field shape correlate with improved electrophoretic resolution. The most critical variable appears to be the angle between the alternate electrical fields. The most effective electrode configurations yield angles of more than 110. A continually increasing angle between the fields produces band sharpening that greatly enhances theresolution.