Immunolocalization of laminin in normal rat liver and biosynthesis of laminin by hepatic lipocytes in primary culture.
Immunolocalization of laminin in normal rat liver and biosynthesis of laminin by hepatic lipocytes in primary culture.
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层粘连蛋白在正常大鼠肝脏中的免疫定位以及原代培养物中肝脂肪细胞的层粘连蛋白的生物合成。
DOI：
10.1016/0016-5085(88)90566-5
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发表时间：
1988
期刊：
影响因子：
29.4
通讯作者：
Bissell,DM
中科院分区：
文献类型：
--
作者：
Maher,JJ;Friedman,SL;Roll,FJ;Bissell,DM
Laminin, a glycoprotein with a molecular weight of ~850,000 daltons, is a major constituent of most epithelial basement membranes. Its presence in the extracellular matrix of normal liver, however, is debated. Using two affinity-purified antibodies directed against laminin, we have localized the glycoprotein within normal rat liver and identified its cellular source. Immunofluorescent staining of rat liver sections revealed laminin in a continuous distribution around hepatic sinusoids, adjacent to hepatocytes and sinusoidal lining cells. To determine the cellular origin of laminin, three perisinusoidal cell populations (hepatocytes, sinusoidal endothelial cells, and lipocytes) were purified from enzymatically dispersed rat liver and were established in primary culture. By immunofluorescence, laminin was associated almost exclusively with lipocytes. Synthesis of laminin was demonstrated by immunoprecipitation of the protein from lipocyte culture medium pulse-labeled with radioactive methionine. These results show that in adult liver, laminin is present in the perisinusoidal matrix and is produced by hepatic lipocytes. Lipocytes, which have the capacity to produce collagen as well as laminin, may be the principal source of extracellular matrix proteins in the perisinusoidal space, and may contribute to subendothelial fibrosis resulting from liver injury.
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影响因子：
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10.1073/pnas.74.11.4835
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影响因子：
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影响因子：
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作者：
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