Comparison of early passage, senescent and hTERT immortalized endothelial cells
Comparison of early passage, senescent and hTERT immortalized endothelial cells
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DOI：
10.1016/j.yexcr.2005.05.002
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发表时间：
2005-09-10
影响因子：
3.7
通讯作者：
Voglauer, R
中科院分区：
文献类型：
--
作者：
Chang, MWF;Grillari, J;Voglauer, R
需要标准化的实验条件，以获得相关的和可重复的结果，增加了良好表征的连续生长的细胞系，表现出其正常对应物的特性的需求。通过异位表达人端粒酶催化亚基（hTERT）使正常人细胞永生化已显示导致高度分化的细胞系。然而，端粒酶活性增加对蛋白表达谱的影响迄今尚未研究。因此，我们通过hTERT过表达永生化人脐静脉内皮细胞（HUVECs），并将其与正常的早期传代和衰老的内皮细胞进行比较。这项研究，包括蛋白质组学方法，表明异位hTERT表达导致稳定生长的细胞系。虽然这些细胞是高度分化的，但细胞系的蛋白质表达谱与正常早期传代和衰老细胞的蛋白质表达谱不同。(c)2005年爱思唯尔公司All rights reserved.
The need for standardized experimental conditions to gain relevant and reproducible results has increased the demand for well characterized continuously growing cell lines that exhibit the characteristics of their normal counterparts. Immortalization of normal human cells by ectopic expression of the catalytic subunit of human telomerase (hTERT) has shown to result in highly differentiated cell lines. However, the influence of the increased telomerase activity on the protein expression profile was not investigated so far. Therefore, we have immortalized human umbilical vein endothelial cells (HUVECs) by hTERT overexpression and compared them to their normal early passage and senescent counterparts. This study, including a proteomic approach, shows that ectopic hTERT expression leads to a stable growing cell line. Although these cells are highly differentiated, the protein expression profile of the cell line is different to that of normal early passage and senescent cells. (c) 2005 Elsevier Inc. All rights reserved.