Proteomic Analysis of Pancreatic Ductal Adenocarcinoma Cells Reveals Metabolic Alterations
Proteomic Analysis of Pancreatic Ductal Adenocarcinoma Cells Reveals Metabolic Alterations
复制标题
DOI：
10.1021/pr101179t
复制
发表时间：
2011-04-01
影响因子：
4.4
通讯作者：
Petricoin, Emanuel F.
中科院分区：
文献类型：
--
作者：
Zhou, Weidong;Capello, Michela;Petricoin, Emanuel F.
在本工作中，我们利用LTQ：Orbitrap对胰腺导管腺癌细胞(PDAC)和正常胰腺管细胞的蛋白质组进行了鉴定，并从每个样本中鉴定了1200多个蛋白质。在光谱计数无标记定量方法的基础上，我们鉴定了与细胞骨架、细胞黏附、转运、转录、翻译以及细胞增殖相关的大量差异表达的代谢酶和蛋白质。数据表明，PDAC的代谢途径发生了改变，这与Warburg效应一致。我们的分析提供了潜在的全面的PDAC代谢图景，这可能作为PDAC新的诊断和治疗的基础。
In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ: Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.