Elevated levels of soluble CD40 ligand (sCD40L) in serum of patients with systemic autoimmune diseases
Elevated levels of soluble CD40 ligand (sCD40L) in serum of patients with systemic autoimmune diseases
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DOI：
10.1016/j.jaut.2006.02.002
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发表时间：
2006-05-01
影响因子：
12.8
通讯作者：
Routsias, John G.
中科院分区：
文献类型：
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作者：
Goules, Andreas;Tzioufas, Athanasios G.;Routsias, John G.
CD40-CD40L共刺激通路参与了系统性红斑狼疮(SLE)、类风湿性关节炎(RA)和干燥综合征(SS)等多种自身免疫性疾病的发生发展。血清中sCD40L水平的升高与SLE的疾病活动性有关。本研究的目的是探讨sCD40L在狼疮性肾炎发生发展中的作用及其与干燥综合征冷球蛋白血症的关系。采用双抗体夹心法检测SLE、RA、SS患者及17例健康献血员血清sCD40L水平。对狼疮性肾炎患者的尿液和SS患者的唾液等受累组织进行生物标本检测。用sCD40L-EL ISA法检测了6例SLE患者(活动性狼疮性肾炎3例，非活动性狼疮性肾炎3例)以及5例SS合并冷球蛋白血症患者、5例SS合并抗Ro或抗La自身抗体患者和5例年龄匹配的健康对照组的配对血清和唾液样本。我们还研究了SS和SLE患者sCD40L水平与几个实验室和临床参数的可能相关性。我们发现SLE和SS患者的血清sCD40L水平显著高于健康对照组。活动期和非活动期肾炎患者的尿液、SS患者和正常人的唾液标本均未检测到sCD40L。可溶性CD40L在SS和SLE患者血清中升高，但其在SLE肾炎和干燥综合征中的可能作用有待进一步研究。(C)2006爱思唯尔有限公司。保留所有权利。
The CD40-CD40L costimulatory pathway is involved in the evolution of many autoimmune diseases including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and Sjogren's syndrome (SS). Increased levels of sCD40L in the serum have been associated with disease activity in SLE. The aim of this study was to investigate the role of sCD40L in the development of lupus nephritis and examine its possible association with cryoglobulinemia in Sjogren's syndrome. We used a 2-site sandwich ELISA to measure the levels of sCD40L in sera, from 64 patients with SLE, RA and SS and 17 healthy blood donors. Biological specimens from the affected tissues such as urine from patients with lupus nephritis and saliva from patients with SS were also tested. In this regard, paired sera and first morning urine samples from 6 SLE patients (3 with active lupus nephritis and 3 with inactive lupus nephritis) were tested with the sCD40L ELISA protocol as well as paired sera and salivary samples from 5 patients with SS and cryoglobulinemia, 5 patients with SS and anti-Ro or anti-La autoantibodies and 5 age-matched healthy control donors. We also examined possible correlations of sCD40L levels with several laboratory and clinical parameters in SS and SLE. We found that sera from SLE and SS patients had significantly higher levels of sCD40L compared to sera from healthy control donors. No sCD40L was detected, in urine samples of patients with either active or inactive nephritis and in salivary samples from SS patients or normal subjects. Soluble CD40L is elevated in sera of SS and SLE patients but further investigation is needed to determine its possible role in SLE nephritis and Sjogren's syndrome. (c) 2006 Elsevier Ltd. All rights reserved.