Distinct role of surface lymphotoxin expressed by B cells in the organization of secondary lymphoid tissues
Distinct role of surface lymphotoxin expressed by B cells in the organization of secondary lymphoid tissues
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DOI：
10.1016/s1074-7613(02)00397-7
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发表时间：
2002-09-01
期刊：
影响因子：
32.4
通讯作者：
Nedospasov, SA
中科院分区：
文献类型：
--
作者：
Tumanov, AV;Kuprash, DV;Nedospasov, SA
为了明确地确定由B细胞表达的光敏素（LT）的功能贡献，我们产生了从B细胞中缺失LT β基因的小鼠（B-LT β KO小鼠）。与全身性LT β缺失相反，在B-LT β KO小鼠中，仅脾脏微结构受到影响，而淋巴结和派尔集合淋巴结（PP）正常，除了PP的大小减小。尽管B-LT β KO脾脏保留了少量的滤泡树突状细胞（FDC），其似乎依赖于T细胞产生的LP β，但对绵羊红细胞的IgG应答显著降低。因此，B-LT β的器官功能几乎完全限于脾脏，在那里它支持正确的淋巴结构，这对有效的体液免疫应答至关重要。
In order to definitively ascertain the functional contribution of lymphotoxin (LT) expressed by B cells, we produced mice with the LTbeta gene deleted from B cells (B-LTbeta KO mice). In contrast to systemic LTbeta deletion, in B-LTbeta KO mice only splenic microarchitecture was affected, while lymph nodes and Peyer's patches (PP) were normal, except for PP's reduced size. Even though B-LTbeta KO spleens retained a small number of follicular dendritic cells (FDC) which appeared to be dependent on LPbeta produced by T cells, IgG responses to sheep red blood cells were markedly reduced. Thus, the organogenic function of B-LTbeta is almost entirely restricted to spleen, where it supports the correct lymphoid architecture that is critical for an effective humoral immune response.