Selective probing of mRNA expression levels within a living cell
Selective probing of mRNA expression levels within a living cell
复制标题
DOI：
10.1063/1.3213343
复制
发表时间：
2009-08-24
影响因子：
4
通讯作者：
Wickramasinghe, H. Kumar
中科院分区：
文献类型：
--
作者：
Nawarathna, D.;Turan, T.;Wickramasinghe, H. Kumar
我们报告一个选择性和非破坏性的测量mRNA（信使核糖核酸）的表达水平在活细胞内。我们首先修改原子力显微镜的尖端，以创建一个锥形的纳米同轴电缆。在该电缆的内电极和外电极之间施加ac（交流电位）产生介电泳力，将mRNA分子吸引向用基因特异性引物预处理的尖端。我们选择性地提取和分析了高（类似于2500）和极低（110）拷贝数的mRNA从活细胞mRNA在不到10秒。
We report on a selective and nondestructive measurement of mRNA (messenger ribonucleic acid) expression levels within a living cell. We first modify an atomic force microscope tip to create a tapered nanoscale coaxial cable. Application of an ac (alternating potential) between the inner and outer electrodes of this cable creates a dielectrophoretic force attracting mRNA molecules toward the tip-end which is pretreated with gene specific primers. We selectively extracted and analyzed both high (similar to 2500) and extremely low (110) copy number mRNA from a living cell mRNA in less than 10 s.