Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs
Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs
复制标题
DOI：
10.21521/mw.5601
复制
发表时间：
2016-12-01
影响因子：
0.4
通讯作者：
Kempisty, Bartosz
中科院分区：
文献类型：
--
作者：
Antosik, Pawel;Jeseta, Michal;Kempisty, Bartosz
卵巢卵泡发生和卵子发生对着床前胚胎的生长发育有重要影响。尽管这两个过程在包括猪在内的几种哺乳动物中被广泛理解，但影响卵母细胞适当成熟能力的因素以及周围体细胞颗粒细胞（GC）和卵丘细胞（CC）的发育能力仍然不完全清楚。本研究旨在探讨生长分化因子9（GDF 9）和整合素（ITGB 1、ITGB 2、ITGB 3和ITGB 4）在不同大小卵泡和不同发育状态供体猪卵母细胞中的表达。通过RT-qPCR测定评估了从循环母猪的中等卵泡（MFCS）、幼年母猪的小卵泡（SFJG）和循环母猪的小卵泡（SFCS）分离的猪卵母细胞中GDF 9、ITGB 1、ITGB 2、ITGB 3和ITGB 4 mRNA的相对丰度。GDF 9在幼龄母猪小卵泡卵母细胞中的表达显著高于其他两组（P < 0.001）。周期母猪中期卵泡中ITGB 1和ITGB 2 mRNA表达显著下调（分别为P < 0.05和P < 0.001）。ITGB 3 mRNA在幼龄母猪小卵泡卵母细胞中的表达显著降低（P < 0.001），而ITGB 4在中等卵泡（周期母猪）和小卵泡（幼龄母猪）卵母细胞中的表达均较低。总之，GDF 9可能被认为是在卵泡发生的早期阶段调节卵泡生长的主要因子。ITGB的表达受供体猪的青春期状态的显著调节，并且不同卵泡大小可能在猪体内卵泡发育过程中对整合素表达起从属作用。
Ovarian folliculogenesis and oogenesis has a significant impact on embryo growth and development in preimplantation stages. Although both processes are widely understood in several species of mammals, including pigs, the factors influencing the proper maturation capability of oocytes, as well as the developmental competence of the surrounding somatic granulosa cells (GCs) and cumulus cells (CCs), are still not entirely known. This study aimed to investigate the expression of growth differentiation factor 9 (GDF9) and integrins (ITGB1, ITGB2, ITGB3 and ITGB4) in porcine oocytes isolated from follicles of various size and donors characterized by different puberty status. The relative abundance of GDF9, ITGB1, ITGB2, ITGB3, and ITGB4 mRNAs in porcine oocytes isolated from medium follicles of cycling sows (MFCS), small follicles of juvenile gilts (SFJG), and small follicles of cycling sows (SFCS) was assessed by an RT-qPCR assay. It was found an increased expression of GDF9 in oocytes isolated from the small follicles of juvenile gilts as compared to the other two groups (P < 0.001). A significant down-regulation of ITGB1 and ITGB2 oocyte mRNAs collected from medium follicles of cycling sows was observed (P < 0.05 and P < 0.001, respectively). The ITGB3 mRNA expression was significantly decreased in oocytes isolated from small follicles of juvenile gilts (P < 0.001), whereas a lower expression of ITGB4 in oocytes from both medium follicles (cycling sows) and small follicles (juvenile gilts) was observed. In conclusion, GDF9 may be recognized as the main factor regulating follicle growth at early stages of folliculogenesis. The expression of ITGBs is significantly regulated by the puberty status of donor pigs, and different follicular sizes may play a subordinate role in integrin expression during in vivo follicle development in pigs.