THE ACTIN GENES OF DROSOPHILA - PROTEIN CODING REGIONS ARE HIGHLY CONSERVED BUT INTRON POSITIONS ARE NOT
THE ACTIN GENES OF DROSOPHILA - PROTEIN CODING REGIONS ARE HIGHLY CONSERVED BUT INTRON POSITIONS ARE NOT
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DOI：
10.1016/0092-8674(81)90506-7
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
DAVIDSON, N
中科院分区：
文献类型：
--
作者：
FYRBERG, EA;BOND, BJ;DAVIDSON, N
用重组DNA方法分离了果蝇肌动蛋白基因的全套序列，并用基因图谱技术和部分核苷酸测序鉴定了各编码区的结构。对这些基因的结构比较得出了几个意想不到的发现。最引人注目的是，在这些基因的蛋白质编码区域内，插入序列的位置不保守。果蝇肌动蛋白基因之一DmA4在第13位甘氨酸密码子内分裂；其余5个基因在类似位置都没有中断。另一个基因DmA6在307位甘氨酸密码子内分裂；至少有两个果蝇肌动蛋白基因在类似的位置没有分裂。果蝇的肌动蛋白基因都没有在密码子4内分裂，而酵母肌动蛋白基因在密码子4中被打断。这6个果蝇肌动蛋白基因编码几种不同的蛋白质，但每个基因的氨基酸序列都与脊椎动物细胞质肌动蛋白相似。这些基因都没有编码一种在初级序列上与脊椎动物骨骼肌肌动蛋白类似的蛋白质。在每个衍生的肌动蛋白氨基酸序列中，启动子蛋氨酸紧随其后的是半胱氨酸残基，而半胱氨酸残基又位于成熟脊椎动物细胞质肌动蛋白氨基末端的3个酸性氨基酸串之前。这些发现是在肌动蛋白基因进化和功能的背景下讨论的。
The entire set of 6 closely related Drosophila actin genes was isolated using recombinant DNA methodology, and the structures of the respective coding regions were characterized by gene mapping techniques and by nucleotide sequencing of selected portions. Structural comparisons of these genes have resulted in several unexpected findings. Most striking is the nonconservation of the positions of intervening sequences within the protein-encoding regions of these genes. One of the Drosophila actin genes, DmA4, is split within a glycine codon at position 13; none of the remaining 5 genes is interrupted in the analogous position. Another gene, DmA6, is split within a glycine codon at position 307; at least 2 of the Drosophila actin genes are not split in the analogous position. None of the Drosophila actin genes is split within codon 4, where the yeast actin gene is interrupted. The 6 Drosophila actin genes encode several different proteins, but the amino acid sequence of each is similar to that of vertebrate cytoplasmic actins. None of the genes encodes a protein comparable in primary sequence to vertebrate skeletal muscle actin. In each of these derived actin amino acid sequences the initiator methionine is directly followed by a cysteine residue, which in turn precedes the string of 3 acidic amino acids characteristic of the amino termini of mature vertebrate cytoplasmic actins. These findings are discussed in the context of actin gene evolution and function.