Isolation and characterization of 15 polymorphic microsatellite markers for the fig-pollinating wasp, Blastophaga nipponica (Hymenoptera: Agaonidae)
Isolation and characterization of 15 polymorphic microsatellite markers for the fig-pollinating wasp, Blastophaga nipponica (Hymenoptera: Agaonidae)
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DOI：
10.1007/s13355-014-0267-x
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发表时间：
2014-04
影响因子：
1.3
通讯作者：
Junko Kusumi;Z. Su
中科院分区：
文献类型：
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作者：
Junko Kusumi;Z. Su
利用双抑制pcr技术，为无花果传粉小蜂（blastophaga nipponicaGrandi）建立了微卫星标记。获得21个候选微卫星位点，其中15个产生可评分模式。利用日本3个自然居群60个个体的遗传变异汇总统计进一步分析了这15个位点的多态性程度。所有15个位点均为多态性，每个位点产生2-27个等位基因。总体观察杂合度（HO）和期望杂合度（HE）分别为0.465和0.631。结果表明，根据该物种的近交趋势，其平均近交系数（FIS）较高，为0.255。这些标记将有助于对该物种种群结构的研究。
We developed microsatellite markers for the fig-pollinating waspBlastophaga nipponicaGrandi using a dual-suppression-PCR technique. Twenty-one candidates of microsatellite loci were obtained, of which 15 yielded scorable patterns. The degree of polymorphism for the 15 loci was further characterized using summary statistics describing the genetic variation in 60 individuals from three natural populations in Japan. All 15 loci were polymorphic and yielded 2–27 alleles per locus. Overall observed heterozygosity (HO) and expected heterozygosity (HE) were 0.465 and 0.631, respectively. As expected, based on the inbreeding tendency of this species, the mean inbreeding coefficient (FIS) was high (= 0.255). These markers will contribute to studies on the population structure of this species.