Importance of the direct fresh examination in the diagnosis of cutaneous sporotrichosis
Importance of the direct fresh examination in the diagnosis of cutaneous sporotrichosis
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DOI：
10.1111/j.1365-4632.2004.02271.x
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发表时间：
2004-11-01
影响因子：
3.6
通讯作者：
Vignale, RA
中科院分区：
文献类型：
--
作者：
Civila, ES;Bonasse, J;Vignale, RA
背景迄今为止，除了少数作者外，直接新鲜检查尚未被认为是诊断皮肤孢子丝菌病的有效的实验室检查方法。脓液是通过对开放的病变进行数字加压，然后用铲子收集，并检查玻片和盖片之间的材料而获得的。结果85.7%的病例直接、新鲜地检出申克孢子丝菌小体，使作者对该病的快速诊断成为可能。细菌培养确诊率为95.2%。结论新鲜检验法快速、简便、成本低，建议以一级公共卫生实验室为重点，系统应用该方法对该病进行初步诊断。
Background To date the direct fresh examination has not been considered, except by a few authors, as a valid laboratory procedure for the diagnosis of cutaneous sporotrichosis.Methods Forty-two patients with cutaneous sporotrichosis (36 lymphangitic and six fixed) were studied. Pus was obtained through digital compression of opened lesions followed by collection with a spatula and examination of the material between the slide and the coverslip. Cultures on Sabouraud's glucose agar slants with cloramphenicol and cicloheximide were made in all cases.Results Direct, fresh examination asteroid bodies of Sporothrix schenckii were found in 85.7% of the cases, which allowed the authors to immediately diagnose the disease. Cultures confirmed the diagnosis in 95.2% of the patients.Conclusions Fastness, simplicity and low cost of the fresh examination lead us to recommend systematic use of the method for primary diagnosis of the disease, mainly at first level public health laboratories.