Development of an ion microbeam system for irradiating single plant cell[s].
Development of an ion microbeam system for irradiating single plant cell[s].
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DOI：
10.2187/bss.17.298
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发表时间：
2003-12-01
期刊：
影响因子：
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通讯作者：
Inoue, Masayoshi
中科院分区：
文献类型：
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作者：
Yokota, Yuichiro;Funayama, Tomoo;Inoue, Masayoshi
为了准确分析离子束的生物效应，研制了一套用于辐照单个植物细胞的离子微束系统。以烟草BY-2原生质体作为单株细胞模型。原生质体在薄琼脂糖基中培养，放在专门设计的辐照容器中，该容器配有CR-39核径迹探测器(100微米厚的薄片)。培养1个月后，未辐照原生质体的集落形成率为22.7+/-6.7%(3个不同实验的平均+/-SE)。原生质体用编程数为18.3 MeV/u的碳离子辐照，这些碳离子已经被20微米的phi微孔准直。辐照后，通过在27℃的13.4M KOH溶液中蚀刻CR-39薄片9h，准确地确定了被离子击中的原生质体内的位置，碳离子微束的命中率为56.9+/-2.4%(7个不同重复的平均+/-SE)。
An ion microbeam system for irradiating single plant cells was developed to analyze exact biological effects of ion beams. Tobacco BY-2 protoplasts were used as a model of single plant cells. Protoplasts were cultured in thin agarose medium on a specially designed irradiation-vessel, which has a CR-39 nuclear track detector (a 100-micrometer thick sheet). The colony formation rate of unirradiated protoplasts was 22.7 +/- 6.7% (mean +/- SE of 3 different experiments) after a month of culture. Protoplasts were irradiated with programmed numbers of 18.3 MeV/u carbon ions that had been collimated by a 20-micrometer phi micro-aperture. After the irradiation, the positions within the protoplasts that were hit with ions were accurately determined by etching the CR-39 sheet in 13.4M KOH solution at 27 degrees centigrade for 9 h. The hit rate of the carbon ion microbeam, i.e., the percent of the ion particles that hit the protoplast that they were aimed at, was 56.9 +/- 2.4% (mean +/- SE of 7 different replications).