Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction
Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction
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DOI：
10.1093/jexbot/53.370.875
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发表时间：
2002-04-01
影响因子：
6.9
通讯作者：
Planchet, E
中科院分区：
文献类型：
--
作者：
Kaiser, WM;Weiner, H;Planchet, E
硝酸还原酶活性的翻译后调节机制（NR，EC 1.6.6.1）进行了简要总结，它表明，通过这种机制，一氧化氮生产通过NR也迅速调制。新的和部分意想不到的调制机制的细节已通过使用免疫技术。NR的磷酸化状态已经用针对菠菜NR的丝氨酸磷酸化动机提出的肽抗体进行了评估。通过免疫共沉淀实验，阐明了14-3-3与磷酸化NR的结合以及Mg ~（2+）在此过程中的作用。NR磷酸化和14-3-3结合在控制NR蛋白水解的作用的数据进行了讨论。其他NR失活蛋白的一个可能的作用也被简要地考虑和蓖麻NR的调节被描述为一个有趣的特殊情况下，不同于“正常”的机制在几个重要方面。
The mechanism of the post-translational modulation of nitrate reductase activity (NR, EC 1.6.6.1) is briefly summarized, and it is shown that by this mechanism nitric oxide production through NR is also rapidly modulated. New and partly unexpected details on the modulation mechanism have been obtained by using immunological techniques. The phosphorylation state of NR has been assessed with peptide antibodies raised against the serine phosphorylation motive of spinach NR. By co-immunoprecipitation experiments, 14-3-3 binding to phospho-NR and the function of Mg2+ in that process has been elucidated. Conflicting data on the role of NR phosphorylation and 14-3-3 binding in controlling NR proteolysis are discussed. A possible role of other NR inactivating proteins is also briefly considered and the regulation of NR of Ricinus communis is described as an interesting special case that differs from the 'normal' mechanism in several important aspects.