Transcription of unrearranged antigen receptor genes in scid mice.
Transcription of unrearranged antigen receptor genes in scid mice.
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scid 小鼠中未重排抗原受体基因的转录。
DOI：
10.1002/j.1460-2075.1988.tb03041.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Bosma,MJ
中科院分区：
文献类型：
--
作者：
Schuler,W;Schuler,A;Lennon,GG;Bosma,GC;Bosma,MJ
scid小鼠突变体严重缺乏淋巴细胞; B或T淋巴细胞谱系的细胞不能通过血清学或功能测定检测到。然而，如图所示，在scid小鼠的淋巴组织中可检测到B细胞免疫球蛋白（IG）恒定区和可变区基因以及T细胞受体（TCR）基因的生殖系转录物，以及B和T谱系特异性λ 5和T3 δ转录物。我们的结论是，B和T谱系定向细胞确实出现在scid小鼠中，并且它们的IG和TCR基因可被参与其重组的酶所接近。这表明scid在抗原受体基因正常进行重排的阶段损害淋巴细胞生成。
The scid mouse mutant is severely deficient in lymphocytes; cells of the B or T lymphocyte lineage cannot be detected by either serological or functional assays. However, as shown here, germ‐line transcripts of B cell immunoglobulin (Ig) constant and variable region genes and of T cell receptor (TCR) genes are detectable in lymphopoietic tissues of scid mice, as well as B and T lineage‐specific lambda 5 and T3 delta transcripts. We conclude that B and T lineage‐committed cells do arise in scid mice and that their Ig and TCR genes are accessible to enzymes involved in their recombination. This suggests that scid impairs lymphopoiesis at the stage at which antigen receptor genes normally undergo rearrangement.