Antibody cross-reactivity with CD46 and lack of cell surface expression suggest that moesin might not mediate measles virus binding
Antibody cross-reactivity with CD46 and lack of cell surface expression suggest that moesin might not mediate measles virus binding
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抗体与 CD46 的交叉反应性和缺乏细胞表面表达表明 moesin 可能不会介导麻疹病毒结合
作者：
P. Devaux;D. Gerlier
从腹水中的抗膜突蛋白抗体的人和啮齿动物细胞的表面的结合被发现平行的CD 46表达水平。用纯化的抗膜突蛋白抗体没有检测到这种反应性，该抗体识别细胞内膜突蛋白。在Western印迹中，发现抗膜突蛋白抗体与溶解的CD 46和重组可溶形式的CD 46反应。抗膜突蛋白抗体还与仅含有麻疹病毒（MV）血凝素结合到CD 46上所需的SCR I和II结构域的CD 46/CD 4分子反应。我们认为，弱的抗膜突蛋白抗体与CD 46的交叉反应解释了这些抗体对MV进入的抑制作用，膜突蛋白不直接参与MV结合。
The binding of antimoesin antibodies from ascites fluids to the surfaces of human and rodent cells was found to parallel the level of CD46 expression. No such reactivity was detected with a purified antimoesin antibody which recognized intracellular moesin. In Western blots, antimoesin antibodies were found to react with solubilized CD46 and a recombinant soluble form of CD46. Antimoesin antibodies also reacted with CD46/CD4 molecules containing only the SCR I and II domains required for measles virus (MV) hemagglutinin binding onto CD46. We suggest that the weak cross-reactivity of antimoesin antibodies with CD46 explains the inhibitory effect of these antibodies on MV entry and that moesin is not directly involved in MV binding.