EFFECT OF COLD-STORAGE ON PLATELET GLYCOPROTEIN IB AND VESICULATION
EFFECT OF COLD-STORAGE ON PLATELET GLYCOPROTEIN IB AND VESICULATION
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DOI：
10.1046/j.1537-2995.1994.34894353465.x
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发表时间：
1994-08-01
期刊：
影响因子：
2.9
通讯作者：
KNUPP, CL
中科院分区：
文献类型：
--
作者：
BODE, AP;KNUPP, CL
背景：浓缩血小板在22℃保存过程中，表面糖蛋白发生变化，上清血浆中出现膜囊泡。冷藏期间这些变化的程度尚不清楚。研究设计和方法：研究了血小板浓缩液中的膜微粒和表面或总糖蛋白 Ib (GPIb) 的变化，将其分成等分试样，在 4 摄氏度或 22 摄氏度下保存 5 天。结果：冷藏血小板显示总 GPIb 损失较多，单克隆抗体与表面 GPIb 的结合略少，并且对瑞斯托菌素的聚集反应降低相对于 22 摄氏度下的配对血小板对照。此外，与对照相比，储存在 4 摄氏度下的血小板在上清液血浆中产生的微粒多 45%，血小板因子 3 活性多 64%。将 4 摄氏度和 22 摄氏度储存的血小板在 37 摄氏度下加热 1 至 4 小时会加剧这些差异。结论：在 4 摄氏度下储存血小板会导致膜囊泡增加并加速 GPIb 损失。这些差异的幅度很小，但可能导致循环中血小板存活率显着降低。
Background: During storage of platelet concentrates at 22 degrees C, changes occur in surface glycoproteins, and membranous vesicles appear in the supernatant plasma. The extent of these changes during refrigerated storage is not known.Study Design and Methods: Membranous microparticles and changes in surface or total glycoprotein Ib (GPIb) were studied in platelet concentrates divided into aliquots stored at either 4 degrees C or 22 degrees C for 5 days.Results: The refrigerated platelets showed greater loss of total GPIb slightly less binding of monoclonal antibodies to surface GPIb, and reduced aggregation response to ristocetin relative to the paired platelet controls at 22 degrees C. Moreover, the platelets stored at 4 degrees C produced 45-percent more microparticles and 64-percent more platelet factor 3 activity in the supernatant plasma than were produced by the controls. These differences were augmented by warming both 4 degrees C- and 22 degrees C-stored platelets at 37 degrees C for 1 to 4 hours.Conclusion: Storage of platelets at 4 degrees C causes increased membrane vesiculation and accelerated loss of GPIb. The magnitude of these differences was small, but it may contribute to marked reductions in platelet survival in circulation.