Effect of Cnidium Lactone on Serum Mutant P53 and BCL-2/BAX Expression in Human Prostate Cancer Cells PC-3 Tumor-Bearing BALB/C Nude Mouse Model.
Effect of Cnidium Lactone on Serum Mutant P53 and BCL-2/BAX Expression in Human Prostate Cancer Cells PC-3 Tumor-Bearing BALB/C Nude Mouse Model.
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DOI：
10.12659/msm.893745
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发表时间：
2015-08-18
期刊：
影响因子：
--
通讯作者：
Huang S
中科院分区：
文献类型：
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作者：
Bi D;Yang M;Zhao X;Huang S
蛇床内酯是一种天然香豆素化合物，可以抑制多种癌细胞增殖并诱导癌细胞凋亡。本实验通过观察蛇床子内酯对前列腺癌裸鼠模型中分子标志物表达的影响，探讨蛇床子内酯诱导前列腺癌细胞凋亡的作用。将30只接种人前列腺癌细胞PC-3的雄性BALB/C裸鼠随机分为阴性对照组、环磷酰胺组（500 mg/Kg）和蛇床子内酯组（280 mg/Kg、140 mg/Kg和70 mg/Kg）。给药后2周称重。隧道法检测裸鼠肿瘤细胞凋亡。ELISA法检测血清AMACR、CD 147、突变型P53、BCL-2和BAX表达水平。分离肿瘤组织并称重。蛇床子内酯3个剂量组小鼠体重无明显变化（p>0.05），环磷酰胺组小鼠体重明显下降（p<0.05）。在接受3种不同剂量的蛇床子内酯的组中，肿瘤重量、CD 147、突变型P53和BCL-2水平显著低于阴性对照组（p<0.05）。其中280 mg/Kg和140 mg/Kg剂量组上述指标较环磷酰胺组明显降低（p<0.05）。蛇床子素组和环磷酰胺组AMACR和BAX水平无显著性差异（p>0.05）。蛇床子内酯可能通过调节CD 147、突变型P53和BCL-2的表达而诱导前列腺癌细胞凋亡并抑制其增殖。
Cnidium lactone is a natural coumarin compound that can inhibit a variety of cancer cell proliferation and induce cancer cell apoptosis. This experiment investigated the effect of cnidium lactone on molecular marker expression in prostate cancer nude mice to study its effect in inducing apoptosis. We randomly and equally divided 30 male BALB/C nude mice inoculated with human prostate cancer cells PC-3 into a negative control group, a cyclophosphamide group (500 mg/Kg), and cnidium lactone groups at 3 doses (280 mg/Kg, 140 mg/Kg, and 70 mg/Kg). The mice were weighed at 2 weeks after administration. Tunnel assay was applied to test the nude mice tumor cell apoptosis. ELISA was performed to detect serum AMACR, CD147, mutant P53, BCL-2, AND BAX expression levels. Tumor tissue was separated and weighed. Mice weight did not change significantly in the groups receiving 3 different doses of cnidium lactone(p>0.05), while it decreased obviously in the cyclophosphamide group (p<0.05). Tumor weight, CD147, mutant P53, and BCL-2 levels were significantly lower in the groups receiving 3 different doses of cnidium lactone than in the negative control group (p<0.05). Among them, the abovementioned indexes decreased markedly in the 280 mg/Kg and 140 mg/Kg dose groups than in the cyclophosphamide group (p<0.05). AMACR and BAX levels showed no significant difference in the cnidium lactone group or the cyclophosphamide group (p>0.05). Cnidium lactone may induce prostate cancer cell apoptosis and inhibit its proliferation through regulating CD147, mutant P53, and BCL-2 expression in nude mice.