Differential expression of Sonic Hedgehog in lung adenocarcinoma and lung squamous cell carcinoma.
Differential expression of Sonic Hedgehog in lung adenocarcinoma and lung squamous cell carcinoma.
复制标题
声波刺猬在肺腺癌和肺鳞癌中的差异表达。
DOI：
10.4149/neo_2018_181228n1002
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发表时间：
2019-06
期刊：
影响因子：
3
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Xu L;Xiong H;Shi W;Zhou F;Zhang M;Hu G;Mei J;Luo S;Chen L
Sonic hedgehog基因（Shh）的过度表达与几种癌症的进展有关。Shh在非小细胞肺癌（NSCLC）中的表达已被报道，但结果不一致。肺腺癌（LAC）和肺鳞状细胞癌（LSCC）是非小细胞肺癌的两个主要亚型，它们具有不同的基因型和临床治疗选择。NSCLC患者标本中Shh的表达情况尚未根据组织学亚型全面确定。采用免疫组化法（IHC）检测167例NSCLC患者（56例LAC患者和111例LSCC患者）的Shh表达水平，并采用Kaplan-Meier法分析患者的无病生存期和总生存期。采用酶联免疫分析法（ELISA）检测肺炎或胸膜脓肿、肺结核、LAC和LSCC患者胸腔积液中Shh蛋白水平。我们发现，与配对的相邻组织相比，LAC和LSCC患者的肿瘤组织中Shh表达均升高，而Shh水平仅在LSCC中与肿瘤分化呈负相关，高Shh表达的LSCC患者预后较差。此外，与肺旁和LAC胸腔积液相比，LSCC患者胸腔积液中的Shh水平升高。肿瘤组织或胸腔积液中的Shh表达可能是LSCC患者的潜在诊断和预后标志物，胸腔积液中的Shh可能有助于区分LAC和LSCC。
Overexpression of Sonic hedgehog (Shh) is associated with progression of several cancers. The expression of Shh in non-small cell lung cancer (NSCLC) has been reported with inconsistent results. Lung adenocarcinoma (LAC) and lung squamous cell carcinoma (LSCC) are two major subtypes of NSCLC, which have different genetic genotypes and clinical therapeutic options. The expression of Shh in specimen of patients with NSCLC has yet to be comprehensively determined according to histological subtypes. Shh expression level was determined in 167 NSCLC patients (56 LAC patients and 111 LSCC patients) by immunohistochemical assay (IHC), and disease-free survival and overall survival of patients were analyzed using the Kaplan-Meier method. Shh protein level in pleural effusion from patients with pneumonia or pleural empyema, tuberculosis, LAC and LSCC was measured with enzyme-linked immunoassay (ELISA). We found that Shh expression is increased in tumor tissues from both LAC and LSCC patients compared with the paired adjacent tissues, while Shh level is negatively correlated with tumor differentiation only in LSCC, LSCC patients containing higher-Shh expression have a poorer prognosis. Furthermore, Shh level is elevated in pleural effusion from LSCC patients compared with that of parapneumonic and LAC pleural effusion. Shh expression in tumor tissues or pleural effusion may represent a potential diagnostic and prognostic marker of LSCC patients, pleural effusion Shh may assist to distinguish between LAC and LSCC.
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影响因子：
120.1
作者：
Daniel Jones
通讯作者：
Daniel Jones
DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
2.9
作者：
Ma Y;Fan M;Dai L;Kang X;Liu Y;Sun Y;Xiong H;Liang Z;Yan W;Chen K
通讯作者：
Chen K
影响因子：
158.5
作者：
Sandler, Alan;Gray, Robert;Johnson, David H.
通讯作者：
Johnson, David H.
影响因子：
8
作者：
Szczepny A;Rogers S;Jayasekara WSN;Park K;McCloy RA;Cochrane CR;Ganju V;Cooper WA;Sage J;Peacock CD;Cain JE;Burgess A;Watkins DN
通讯作者：
Watkins DN