Effects of oestrogen deficiency on osteoclastogenesis in the rat periodontium
Effects of oestrogen deficiency on osteoclastogenesis in the rat periodontium
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DOI：
10.1016/s0003-9969(01)00086-3
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发表时间：
2002-01-01
影响因子：
3
通讯作者：
Ozawa, H
中科院分区：
文献类型：
--
作者：
Kawamoto, S;Ejiri, S;Ozawa, H
本研究旨在研究卵巢切除术后大鼠牙周组织中破骨细胞生成的定量变化。 9 周龄的 Wistar 大鼠接受卵巢切除术 (OVX) 或假手术。将渗透泵植入 24 只大鼠体内，并连续输注 17β-雌二醇或载体溶液。将大鼠分配至以下组之一：(1)OVX+媒介物； (2) 假手术+载体；或(3) OVX+17β-雌二醇。术后第7天和第14天，每组处死4只大鼠。将下颌骨脱矿并包埋在石蜡中。切下第一磨牙区域牙槽骨的正面切片进行酶组织化学和免疫组织化学分析。第7天，两组骨表面的抗酒石酸酸性磷酸酶（TRAP）阳性细胞数量没有显着差异。然而，在骨表面前分离的 TRAP 阳性单核细胞的数量在第 1 组中显着高于第 2 组和第 3 组。在第 14 天，第 1 组中附着在骨表面上的 TRAP 阳性细胞的数量显着高于第 7 天的情况。与第 2 组和第 3 组相比，第 14 天，第 1 组中附着在骨上的 TRAP 阳性细胞的细胞核数量也显着增加。这些发现表明雌激素缺乏会诱导大鼠牙周组织中的破骨细胞生成，并且输注 E2 可以预防破骨细胞生成的数量变化。 (C) 2002 年，爱思唯尔科学有限公司出版。
This study was designed to investigate quantitative changes in osteoclast generation in rat periodontium following ovariectomy. Wistar rats, aged 9 weeks, were subjected to either ovariectomy (OVX) or sham surgery. Osmotic pumps were implanted in 24 rats and either 17beta-oestradiol or vehicle solution were infused continuously. The rats were assigned to one of the following groups: (1) OVX + vehicle; (2) sham + vehicle; or (3) OVX+ 17beta-oestradiol. On the days 7 and 14 after surgery, four rats in each group were killed. Mandibles were demineralized and embedded in paraffin. Frontal sections of alveolar bone in the region of the first molar were cut for enzyme histochemistry and immunohistochemistry. On day 7, there was no significant difference in the number of tartrate-resistant acid phosphatase (TRAP)-positive cells located on bone surfaces in either group. However, the number of TRAP-positive mononuclear cells that were separated front the bone surface was significantly higher in group I than in groups 2 and 3. On the day 14, the number of TRAP-positive cells in group 1, which were attached to the bone surface, was significantly higher than had been apparent on day 7. There were also significant increases in the number of nuclei of TRAP-positive cells attached to the bone in group I compared with groups 2 and 3 on day 14. These findings demonstrate that oestrogen deficiency induces of osteoclastogenesis in the rat periodontium and that quantitative changes in osteoclastogenesis could be prevented by E2 infusion. (C) 2002 Published by Elsevier Science Ltd.