The first report on the molecular identification of Termitomyces of Central Luzon, Philippines
The first report on the molecular identification of Termitomyces of Central Luzon, Philippines
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菲律宾中吕宋地区鸡蚁分子鉴定首次报告
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
J. Undan
中科院分区：
文献类型：
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作者：
R. Reyes;Jesusa Q. Undan;R. M. Dulay;S. Kalaw;J. Undan
按照分离、扩增和测序DNA片段的标准方法，从菲律宾吕宋岛中部自然栖息地(白蚁丘)采集的白蚁菌子实体中提取DNA样本。用ITS3R和ITS4D引物对样本的rDNA-ITS区进行聚合酶链式反应(PCR)扩增，然后进行测序和BLAST分析，并与现有的NCBI数据进行比较。鉴定出球囊拟青霉(T.Bulborhizus)和乳白拟青霉(T.clypeatus)两种。与NCBI报道的其他白蚁相比，不同样本的rDNA-ITS序列产生的百分比值较低，球孢子虫的百分率为92%，克氏锥虫的百分率为87%。
DNA samples from the fruiting bodies of Termitomyces which were previously collected from their natural habitats (termite mounds) in Central Luzon, Philippines were extracted following the standard protocol on the isolation, amplification and sequencing of DNA fragments. The rDNA-ITS regions of the samples were PCR amplified using ITS3R and ITS4D primer and subsequently sequenced and analyzed using BLAST and compared with the existing NCBI data. Two species of Termitomyces namely T. bulborhizus and T. clypeatus were identified. The rDNA-ITS sequences of the different samples yielded lower percentage value i.e. 92% for T. bulborhizus and 87% for T. clypeatus when compared to other NCBI reported Termitomyces.