Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo
Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo
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DOI：
10.3748/wjg.14.1858
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发表时间：
2008-03-28
影响因子：
4.3
通讯作者：
Hou, Ya-Yi
中科院分区：
文献类型：
--
作者：
Wang, Qing-Ling;Wang, Hui;Hou, Ya-Yi
目的：通过体外和体内模型探讨midkine （MK）及其截断形式（tMK）是否参与胃肿瘤的发生。方法：构建人MK和tMK质粒，在胃腺癌细胞BGC823中表达，研究过表达MK或tMK对裸鼠细胞生长和发生的影响。结果：与对照细胞相比，转染mk或转染tmk的细胞生长明显增加，且转染tmx的细胞生长速度快于转染mk的细胞。转染MK或tMK基因的细胞集落形成数量大于对照细胞。转染mk或转染tmk细胞的裸鼠比未转染任何一种基因的裸鼠更早观察到可见肿瘤，肿瘤组织的大小和重量也更大。结论：过表达的MK或tMK在体外和体内均能促进人胃癌细胞生长，且tMK的作用大于MK， tMK可能是较MK更有希望治疗恶性肿瘤的基因治疗靶点。(c) 2008 WJG。版权所有。
AIM: To determine whether midkine (MK) and its truncated form (tMK) contribute to gastric tumorigenesis using in vitro and in vivo models.METHODS: Human MK and tMK plasmids were constructed and expressed in BGC823 (a gastric adenocarcinoma cell line) to investigate the effect of over-expressed MK or tMK on cell growth and turmorigenesis in nude mice.RESULTS: The growth of MK-transfected or tMK-transfected cells was significantly increased compared with that of the control cells, and tMX-transfectecl cells grew more rapidly than MK-transfected cells. The number of colony formation of the cells transfected with MK or tMK gene was larger than the control cells. In nude mice injected with MK-transfected or tMK-transfected cells, visible tumor was observed earlier and the tumor tissues were larger in size and weight than in control animals that were injected with cells without the transfection of either genes.CONCLUSION: Over-expressed MK or tMK can promote human gastric cancer cell growth in vitro and in vivo, and tMK has greater effect than MK. tMK may be a more promising gene therapeutic target compared with MK for treatment of malignant tumors. (c) 2008 WJG. All rights reserved.