Continuous lactic acid fermentation using a plastic composite support biofilm reactor
Continuous lactic acid fermentation using a plastic composite support biofilm reactor
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DOI：
10.1007/s002530100820
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发表时间：
2001-12-01
影响因子：
5
通讯作者：
Gvozdenovic-Jeremic, J
中科院分区：
文献类型：
--
作者：
Cotton, JC;Pometto, AL;Gvozdenovic-Jeremic, J
采用固定化细胞生物膜反应器对干酪乳杆菌乳酸亚种进行连续发酵。鼠李糖酵母（ATCC 11443）。在爱荷华州州立大学，一种独特的塑料复合材料支持（PCS），刺激生物膜的形成已经开发出来。乳酸菌的优化PCS共混物含有50%（wt/wt）农产品[35%（wt/wt）大豆皮粉、5%（wt/wt）大豆粉、5%（wt/wt）酵母提取物、5%（wt/wt）干牛白蛋白和矿物盐]和50%（wt/wt）通过高温挤出生产的聚丙烯（PP）。PCS管的壁厚为3.5 mm，外径为10.5 mm，并切成10 cm长。将六个PCS管（三排两个平行管）以网格方式结合到新玩法Bioflo 3000发酵罐的1.2-1容器的搅拌器轴上。PCS刺激生物膜形成，为附着和悬浮的细胞提供营养，并增加乳酸产生。PCS管上的生物膜厚度通过搅拌速度控制。PCS生物膜反应器和PP控制反应器在0.4 h（-1）稀释速率和125 rpm搅拌下分别实现了9.0和5.8 g l（-1）h（-1）的最佳平均生产速率，产率约为70%。
An immobilized-cell biofilm reactor was used for the continuous production of lactic acid by Lactobacillus casei subsp. rhamnosus (ATCC 11443). At Iowa State University, a unique plastic composite support (PCS) that stimulates biofilm formation has been developed. The optimized PCS blend for Lactobacillus contains 50% (wt/wt) agricultural products [35% (wt/wt) ground soy hulls, 5% (wt/wt) soy flour, 5% (wt/wt) yeast extract, 5% (wt/wt) dried bovine albumin, and mineral salts] and 50% (wt/wt) polypropylene (PP) produced by high-temperature extrusion. The PCS tubes have a wall thickness of 3.5 mm, outer diameter of 10.5 mm, and were cut into 10-cm lengths. Six PCS tubes, three rows of two parallel tubes, were bound in a grid fashion to the agitator shaft of a 1.2-1 vessel for a New Brunswick Bioflo 3000 fermentor. PCS stimulates biofilm formation, supplies nutrients to attached and suspended cells, and increases lactic acid production. Biofilm thickness on the PCS tubes was controlled by the agitation speed. The PCS biofilm reactor and PP control reactor achieved optimal average production rates of 9.0 and 5.8 g l(-1) h(-1), respectively, at 0.4 h(-1) dilution rate and 125-rpm agitation with yields of approximately 70%.