Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa
Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa
复制标题
DOI：
10.1007/s00705-008-0301-9
复制
发表时间：
2009-02-01
影响因子：
2.7
通讯作者：
Valkonen, Jari P. T.
中科院分区：
文献类型：
--
作者：
Mbanzibwa, D. R.;Tian, Y. P.;Valkonen, Jari P. T.
对来自乌干达和坦桑尼亚维多利亚湖盆地的6株木薯褐条病毒（CBSV， Ipomovirus属；potyvirus科）进行了鉴定。病毒颗粒长650 nm。完整外壳蛋白（CP）编码序列（1101个核苷酸，nt）在nt和氨基酸（aa）水平上的同源性分别为90.7 ~ 99.5%和93.7 ~ 99.5%。3'未翻译区长度分别为225、226和227 nt。与之前在坦桑尼亚和莫桑比克沿海低地鉴定的6株CBSV分离株的部分CP序列（714 nt）相比，这8株分离株仅75.8 ~ 77.5% （nt）和87.0 ~ 89.9% （aa）相同。因此，东非存在两个基因不同且地理上分离的CSBV种群。
Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7-99.5 and 93.7-99.5% identical at the nt and amino acid (aa) levels, respectively. The 3' untranslated region was 225, 226 or 227 nt long. These eight isolates were only 75.8-77.5% (nt) and 87.0-89.9% (aa) identical when compared to the partial CP sequences (714 nt) of six CBSV isolates characterized previously from the costal lowlands of Tanzania and Mozambique. Hence, two genetically different and geographically separated populations of CSBV exist in East Africa.