Near-infrared fluorescent proteins engineered from bacterial phytochromes.
Near-infrared fluorescent proteins engineered from bacterial phytochromes.
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近红外荧光蛋白由细菌植物色素设计。
DOI：
10.1016/j.cbpa.2015.06.005
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发表时间：
2015-08
影响因子：
7.8
通讯作者：
Verkhusha VV
中科院分区：
文献类型：
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作者：
Shcherbakova DM;Baloban M;Verkhusha VV
近红外荧光蛋白(NIR FP)、可光激活的NIR FP和由细菌光敏色素光受体(BphPs)发展而来的蛋白质-蛋白质相互作用的近红外报告蛋白(NIR FFP)具有先进的无创深部组织成像技术。在这里，我们提供了BphP衍生的近红外探测器的简要指南，并重点介绍了它们在体内的应用。我们描述了近红外荧光蛋白的表型以及它们的光化学和细胞内特性。我们讨论了近红外FP在基础研究和翻译研究中用于各种细胞类型、组织和动物模型成像的应用。在这一讨论中，我们关注的是近红外荧光蛋白，它能有效地整合内源性胆绿素发色团，因此可以像绿色荧光蛋白一样直接使用。我们还概述了近红外荧光在不同成像平台上的使用，从平面荧光到层析和光声技术。
Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.