Proteomic identification of target proteins in normal but nonfertilizing sperm
Proteomic identification of target proteins in normal but nonfertilizing sperm
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DOI：
10.1016/j.fertnstert.2014.04.039
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发表时间：
2014-08-01
影响因子：
6.7
通讯作者：
Mandelbaum, Jacqueline
中科院分区：
文献类型：
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作者：
Frapsauce, Cynthia;Pionneau, Cedric;Mandelbaum, Jacqueline
目的：确定IVF失败的男性分子原因（精子与透明卵母细胞结合缺陷，无任何明显的卵母细胞异常），这是经典精子分析无法检测到的。设计：病例对照前瞻性研究。设置：大学医院。患者：我们比较了完全受精失败和没有精子与透明膜结合的患者精子的蛋白质组谱，控制干预：所有样本根据其等电点分为三个部分后，通过二维荧光差异凝胶电泳（2D-DIGE）进行分析。主要结果测量：通过质谱法鉴定不育男性和对照组之间差异表达的蛋白质。结果：结论：2D-DIGE技术可作为IVF不孕症诊断和预后的一个新靶点。（（C）2014年由美国生殖医学学会。
Objective: To identify the male molecular causes of failures of IVF (with a deficient binding of spermatozoa to the zona pellucida, without any obvious oocyte anomaly), which are undetected by classical sperm analysis.Design: Case-control prospective study.Setting: University hospital.Patient(s): Proteomic profiles of spermatozoa in patients with a complete failure of fertilization and no spermatozoa bound to the zona pellucida were compared with those of controls (men with normal fertilization and cleavage rates after classical IVF for tubal indication).Intervention(s): All samples were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) after being divided into three fractions according to their isoelectric point.Main Outcome Measure(s): Differentially expressed proteins between infertile men and controls were identified by mass spectrometry.Result(s): Seventeen proteins differentially expressed between cases and controls were found. Twelve of these proteins were identified by mass spectrometry, and two may influence gametes interaction: laminin receptor LR67 and L-xylulose reductase (P34H).Conclusion(s): This study shows that 2D-DIGE might be useful in finding potential targets for diagnosis and prognosis of idiopathic infertility in IVF. ((C)2014 by American Society for Reproductive Medicine.)